Anti-SHROOM4 Antibody Picoband™

Product Name

Anti-SHROOM4 Antibody Picoband™

View all SHROOM4 Antibodies

SKU/Catalog Number

A10866

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SHROOM4 Antibody Picoband™ catalog # A10866. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SHROOM4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10866)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SHROOM4 recombinant protein (Position: Y262-H928).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10866 is reactive to SHROOM4 in Human

Applications

A10866 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

170-180 kDa

Calculated molecular weight

164.857kDa

Background of SHROOM4

This gene encodes a member of the APX/Shroom family, which contain an N-terminal PDZ domain and a C-terminal ASD2 motif. The encoded protein may play a role in cytoskeletal architecture. Mutations in this gene have been linked to the X-linked Stocco dos Santos syndrome characterized by cognitive disabilities. Alternatively spliced transcript variants have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SHROOM4 using anti-SHROOM4 antibody (A10866).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human U-87 MG whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM4 antigen affinity purified polyclonal antibody (Catalog # A10866) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM4 at approximately 170-180 kDa. The expected band size for SHROOM4 is at 165 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-SHROOM4 antibody (A10866).
Overlay histogram showing RT4 cells stained with A10866 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHROOM4 Antibody (A10866, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-SHROOM4 antibody (A10866).
Overlay histogram showing U20S cells stained with A10866 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHROOM4 Antibody (A10866, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SHROOM4 (Source: Uniprot.org, NCBI)

Alternative Names

Protein Shroom4 SHROOM4 SHAP, shrm4 shroom family member 4 protein Shroom4|second homolog of apical protein

For more info on SHROOM4, check out the SHROOM4 Infographic

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In this infographic, you will see the following information for SHROOM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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