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Product Info Summary
| SKU: | A04515 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SIK2 Antibody
SKU/Catalog Number
A04515
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIK2 Antibody (Catalog # A04515). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SIK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SIK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04515)
Host
Rabbit
Contents
SIK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SIK2 antibody was raised against a 16 amino acid synthetic peptide near the center of human SIK2. The immunogen is located within amino acids 270 - 320 of SIK2.
Cross-reactivity
SIK2 antibody is predicted to not cross-react with other SIK protein family members.
Reactive Species
A04515 is reactive to SIK2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
103.9 kDa
Background of SIK2
Salt-inducible kinase 2 (SIK2), like its closely related homolog SIK1, belongs AMPK subfamily of the Ser/Thr protein kinase family and negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1 alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04515 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SIK2 antibody can be used for detection of SIK2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.
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Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.
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Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.
Specific Publications For Anti-SIK2 Antibody (A04515)
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