Product Info Summary
SKU: | A04436-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SIM2 Antibody Picoband™
SKU/Catalog Number
A04436-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIM2 Antibody Picoband™ catalog # A04436-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SIM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04436-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human SIM2 recombinant protein (Position: Y337-R609). Human SIM2 shares 85.8% amino acid (aa) sequence identity with mouse SIM2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04436-2 is reactive to SIM2 in Human, Monkey, Mouse, Rat
Applications
A04436-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
73.219kDa
Background of SIM2
Single-minded homolog 2 is a protein that in humans is encoded by the SIM2 gene. This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SIM2 using anti-SIM2 antibody (A04436-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: rat skeletal muscle tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIM2 antigen affinity purified polyclonal antibody (Catalog # A04436-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIM2 at approximately 65 kDa. The expected band size for SIM2 is at 73 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of PC-3 cells using anti-SIM2 antibody (A04436-2).
Overlay histogram showing PC-3 cells stained with A04436-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIM2 Antibody (A04436-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SIM2 (Source: Uniprot.org, NCBI)
Gene Name
SIM2
Full Name
Single-minded homolog 2
Weight
73.219kDa
Alternative Names
human transcription factor SIM2, homolog of the Drosophila single-minded geneSIM110BHLHE15; short isoform; single-minded (Drosophila) homolog 2; single-minded homolog 2 (Drosophila); transcription factor SIM2 SIM2 HMC13F06, HMC29C01, SIM, bHLHe15 SIM bHLH transcription factor 2 single-minded homolog 2|class E basic helix-loop-helix protein 15|single-minded family bHLH transcription factor 2|transcription factor SIM2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIM2, check out the SIM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIM2 Antibody Picoband™ (A04436-2)
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