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SIRT1 antibody (A00018-1) performs well in ELISA, IF, ICC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 20 Publications

a00018 1 sirt1 primary antibodies if testing 3

IF analysis of SIRT1 using anti-SIRT1 antibody (A00018-1) and anti-Beta Tubulin antibody (M01857-3).
SIRT1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIRT1 Antibody (A00018-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-SIRT1 Antibody Picoband®

SIRT1 antibody

20 Publications

Reactive Species	Host	Application
Human	Rabbit	ELISA, IF, ICC, WB

Tested Applications

SIRT1 antibody was positively detected by WB in:

human Hela whole cell, human 293T whole cell, human K562 whole cell, human MCF-7 whole cell, human SiHa whole cell, human A549 whole cell, human CACO-2 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

SIRT1 antibody was positively detected by ICC/IF in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Human

Tested

Recommend

Monkey

Predicted

Dog

Predicted

Rat

Predicted

Rabbit

Predicted

Sheep

Predicted

Mouse

Predicted

Product Information

Product Name

Anti-SIRT1 Antibody Picoband®
View all SIRT1 Antibodies

Form

Lyophilized

Uniprot

Q96EB6

Gene ID

23411

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SIRT1 recombinant protein (Position: Y142-H533).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

120 kDa

Calculated Molecular Weight

81.7 kDa

Background of SIRT1

Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

Cite This Product

Anti-SIRT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00018-1)

Protocols

Western Blot protocol for SIRT1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SIRT1 antibody

Review Protocol

Publications

Yangjingzhongyu Decoction regulates primordial follicle initiation via LncRNA-Smad1/AMPK dual pathways in diminished ovarian reserve

Published: 2026-01-05

Journal: Journal of Ovarian Research

Application: WB

Impact Factor: 4.20

Species: Rat

PMID: 41491705

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Anti-SIRT1 Antibody Picoband®

SIRT1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Yangjingzhongyu Decoction regulates primordial follicle initiation via LncRNA-Smad1/AMPK dual pathways in diminished ovarian reserve

Dapagliflozin treatment ameliorates oxidative stress, apoptosis and inflammation in tenofovir-induced nephrotoxicity in rats

Chondroitin sulfate A-selenium nanoparticles protect chondrocytes from T-2 toxin-induced oxidative stress and mitochondrial dysfunction through activating autophagy by the SIRT1-AMPK-FOXO3 pathway

Protective Effect of FABP5 Against 6-OHDA Induced Parkinson’s Disease Via PPARγ/SIRT1/PGC-1α Signaling Pathway

TNF-α-downregulated SIRT1 regulates mitochondrial-autophagy and apoptosis in renal vascular endothelial cells involved in trichloroethylene-induced immune kidney injury

Fucoidan Improves D-Galactose-Induced Cognitive Dysfunction by Promoting Mitochondrial Biogenesis and Maintaining Gut Microbiome Homeostasis

Phoenixin 20 ameliorates pulmonary arterial hypertension via inhibiting inflammation and oxidative stress

Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control

Resveratrol attenuates cigarette smoke extract induced cellular senescence in human airway epithelial cells by regulating the miR-34a/SIRT1/NF-κB pathway

Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora

Trichloroethylene induces immune renal tubular injury through SIRT 1/HSP 70/TLR 4 pathway in BALBc mice

Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model

The inhibitory effects of Dulaglutide on cellular senescence against high glucose in human retinal endothelial cells

DJ-1 activates the Atg5-Atg12-Atg16L1 complex via Sirt1 to influence microglial polarization and alleviate cerebral ischemia/reperfusion-induced inflammatory injury

Transcranial Photobiomodulation Therapy Ameliorates Perioperative Neurocognitive Disorder Through Modulation of Mitochondrial Function in Aged Mice

GM1 Ameliorates Lead-Induced Cognitive Deficits and Brain Damage Through Activating the SIRT1/CREB/BDNF Pathway in the Developing Male Rat Hippocampus

Resveratrol prevents cognitive deficits induced by chronic unpredictable mild stress: Sirt1/miR-134 signalling pathway regulates CREB/BDNF expression in hippocampus in vivo and in vitro

Resveratrol suppresses hyperoxia-induced nucleocytoplasmic shuttling of SIRT1 and ROS production in PBMC from preterm infants in vitro

Dietary restriction reduces blood lipids and ameliorates liver function of mice with hyperlipidemia

Dietary quercetin ameliorates nonalcoholic steatohepatitis induced by a high-fat diet in gerbils

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