Anti-SIRT6 Monoclonal Antibody

Product Name

Anti-SIRT6 Monoclonal Antibody

View all Sirt6 Antibodies

SKU/Catalog Number

M00611-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-SIRT6 Monoclonal Antibody catalog # M00611-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SIRT6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00611-2)

Host

Mouse

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

6C9-D10-D3

Isotype

IgG

Immunogen

Purified recombinant human SIRT6 protein expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00611-2 is reactive to Sirt6 in Human, Monkey, Mouse, Rat

Applications

M00611-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36.92kDa

Background of Sirt6

CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-2000
ELISA 1:10000-20000

Validation Images & Assay Conditions

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Figure 2. Immunofluorescent staining data of SIRT6 using Anti-SIRT6 Monoclonal Antibody (M00611-2).

Immunofluorescent analysis of Hela cells fixed by 4% paraformaldehyde and using SIRT6 mouse mAb (dilution 1:100).

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Figure 3. IP validation of SIRT6 using Anti-SIRT6 Monoclonal Antibody (M00611-2).

Immunoprecipitation analysis of Hela cell lysates using SIRT6 mouse mAb.

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Figure 1. Western blotting validation for Anti-SIRT6 Monoclonal Antibody M00611-2

Western blot analysis of extracts from Jurkat

Gene/Protein Information For Sirt6 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.-; NAD-dependent deacetylase sirtuin-6; SIR2L6sir2-related protein type 6; SIR2-like protein 6; sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6; sirtuin 6; sirtuin type 6 Sirt6|2810449N18Rik, AI043036, Sir2l6|sirtuin 6|NAD-dependent protein deacetylase sirtuin-6|NAD-dependent deacetylase sirtuin-6|SIR2-like protein 6|regulatory protein SIR2 homolog 6

For more info on Sirt6, check out the Sirt6 Infographic

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In this infographic, you will see the following information for Sirt6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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