Anti-Homeobox protein SIX5 SIX5 Antibody

Six5 antibody

Boster Bio Anti-Homeobox protein SIX5 SIX5 Antibody (Catalog # A06210). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A06210
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Homeobox protein SIX5 SIX5 Antibody

View all Six5 Antibodies

SKU/Catalog Number

A06210

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Homeobox protein SIX5 SIX5 Antibody (Catalog # A06210). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Homeobox protein SIX5 SIX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06210)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SIX5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06210 is reactive to Six5 in Human, Mouse

Applications

A06210 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

73.717kDa

Background of Six5

Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter By similarity. Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3 By similarity.

Boucher C.A., Hum. Mol. Genet. 4:1919-1925(1995).
Grimwood J., Nature 428:529-535(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Six5 (Source: Uniprot.org, NCBI)

Gene Name

Six5

Full Name

Homeobox protein SIX5

Weight

73.717kDa

Superfamily

SIX/Sine oculis homeobox family

Alternative Names

BOR2; DM locus-associated homeodomain protein; DMAHPsine oculis homeobox homolog 5 (Drosophila); dystrophia myotonica-associated homeodomain protein; homeobox protein SIX5; sine oculis homeobox (Drosophila) homolog 5; Sine oculis homeobox homolog 5; SIX homeobox 5 Six5|Dma, Dmahp, MDMA, MDMAHP, TrexB, TrexBF|sine oculis-related homeobox 5|homeobox protein SIX5|DM locus-associated homeodomain protein homolog|sine oculis homeobox homolog 5|sine oculis-related homeobox 5 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Six5, check out the Six5 Infographic

Six5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Six5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Homeobox protein SIX5 SIX5 Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-SIX5 antibody A06210. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-26

Question

We are currently using anti-SIX5 antibody A06210 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

The anti-SIX5 antibody (A06210) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-02

Question

Will anti-SIX5 antibody A06210 work for WB with eye?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

According to the expression profile of eye, SIX5 is highly expressed in eye. So, it is likely that anti-SIX5 antibody A06210 will work for WB with eye.

Boster Scientific Support

Answered: 2019-08-21

Question

I see that the anti-SIX5 antibody A06210 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-12

Question

I would like to test anti-SIX5 antibody A06210 on mouse eye for research purposes, then I may be interested in using anti-SIX5 antibody A06210 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

The products we sell, including anti-SIX5 antibody A06210, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-14

Question

Would A06210 anti-SIX5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-01

Answer

You can see on the product datasheet, A06210 anti-SIX5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-01

Question

Is there a BSA free version of anti-SIX5 antibody A06210 available?

K. Edwards

Verified customer

Asked: 2013-01-08

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SIX5 antibody A06210 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-01-08

Order DetailsPrice
A06210-100uL

100uL

$360

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A06210
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