Click image to see more details
Product Info Summary
| SKU: | A01062 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Ski oncogene Ski Antibody
SKU/Catalog Number
A01062
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ski oncogene Ski Antibody (Catalog # A01062). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
Ski antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Ski oncogene Ski Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01062)
Host
Rabbit
Contents
Ski Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski.
Reactive Species
A01062 is reactive to SKI in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
80.0 kDa
Background of SKI
TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01062 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
Specific Publications For Anti-Ski oncogene Ski Antibody (A01062)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Ski oncogene Ski Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Ski oncogene Ski Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
