Anti-SLAM/CD150/SLAMF1 Antibody Picoband™

Product Name

Anti-SLAM/CD150/SLAMF1 Antibody Picoband™

View all SLAM/CD150 Antibodies

SKU/Catalog Number

A03988-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLAM/CD150/SLAMF1 Antibody Picoband™ catalog # A03988-2. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SLAM/CD150/SLAMF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03988-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLAM/CD150/SLAMF1 recombinant protein (Position: K34-N213).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03988-2 is reactive to SLAMF1 in Human

Applications

A03988-2 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

37.231kDa

Background of SLAM/CD150

Signaling lymphocytic activation molecule 1 is a protein that in humans is encoded by the SLAMF1 gene. SLAMF1 Antibody: The signaling lymphocyte-activation molecule family member 1 (SLAMF1) is a novel receptor on T cells that potentiates T cell expansion in a CD28-independent manner. SLAMF1 is predominantly expressed by hematopoietic tissues. Reports suggest that the extracellular domain of SLAMF1 is the receptor for the measles virus and acts as a co-activator on both T and B cells. It is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations of the SLAM associated gene may be associated with X-linked lympho-proliferative disease (XLP).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. IHC analysis of SLAM/CD150/SLAMF1 using anti-SLAM/CD150/SLAMF1 antibody (A03988-2).
SLAM/CD150/SLAMF1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLAM/CD150/SLAMF1 Antibody (A03988-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 2. Flow Cytometry analysis of U937 cells using anti-SLAM/CD150/SLAMF1 antibody (A03988-2).
Overlay histogram showing U937 cells stained with A03988-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLAM/CD150/SLAMF1 Antibody (A03988-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SLAMF1 (Source: Uniprot.org, NCBI)

Alternative Names

CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1 SLAMF1 CD150, CDw150, SLAM signaling lymphocytic activation molecule family member 1 signaling lymphocytic activation molecule|IPO-3|SLAM family member 1

For more info on SLAMF1, check out the SLAMF1 Infographic

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In this infographic, you will see the following information for SLAMF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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