Anti-SLC19A1 Antibody Picoband™

Product Name

Anti-SLC19A1 Antibody Picoband™

View all Reduced Folate Carrier/SLC19A1 Antibodies

SKU/Catalog Number

PB9504

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC19A1 Antibody Picoband™ catalog # PB9504. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SLC19A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9504)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SLC19A1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9504 is reactive to SLC19A1 in Human, Monkey, Mouse, Rat

Applications

PB9504 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

64.868kDa

Background of Reduced Folate Carrier/SLC19A1

Solute carrier family 19 (folate transporter), member 1, also known as SLC19A1 or RFC1, is a protein which in humans is encoded by the SLC19A1 gene. Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the method of folate uptake in certain cell types. Methotrexate (MTX) is an antifolate chemotherapeutic agent that is actively transported by the carrier-mediated uptake system. And RFC1 plays a role in maintaining intracellular concentrations of folate.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SLC19A1 using anti-SLC19A1 antibody (PB9504).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse testicular tissue lysates,
Lane 5: mouse ovary tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates,
Lane 7: human placenta tissue lysates,
Lane 8: human HEK293 whole cell lysates,
Lane 9: monkey COS-7 whole cell lysates,
Lane 10: human PC-3 whole cell lysates,
Lane 11: human 22RV1 whole cell lysates,
Lane 12: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC19A1 antigen affinity purified polyclonal antibody (Catalog # PB9504) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC19A1 at approximately 65KD. The expected band size for SLC19A1 is at 65KD.

Gene/Protein Information For SLC19A1 (Source: Uniprot.org, NCBI)

Alternative Names

CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1 SLC19A1 CHMD, FOLT, IFC-1, IFC1, REFC, RFC, RFC1, RFT-1, hRFC, hSLC19A1 solute carrier family 19 member 1 reduced folate transporter|folate transporter 1|intestinal folate carrier 1|placental folate transporter|reduced folate carrier 1|reduced folate carrier protein|reduced folate transporter 1|solute carrier family 19 (folate transporter), member 1

For more info on SLC19A1, check out the SLC19A1 Infographic

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In this infographic, you will see the following information for SLC19A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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