Product Info Summary
SKU: | A09457 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ADP/ATP translocase 3 SLC25A6 Antibody
SKU/Catalog Number
A09457
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ADP/ATP translocase 3 SLC25A6 Antibody (Catalog # A09457). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADP/ATP translocase 3 SLC25A6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09457)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SLC25A6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09457 is reactive to SLC25A6 in Human
Reconstitution
Calculated molecular weight
32866 MW
Background of SLC25A6
Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Cozens A.L., J. Mol. Biol. 206:261-280(1989).
Zhou J., Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
Houldsworth J., Proc. Natl. Acad. Sci. U.S.A. 85:377-381(1988).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09457 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells and 293 cells, using SLC25A6 antibody A09457.
Protein Target Info & Infographic
Gene/Protein Information For SLC25A6 (Source: Uniprot.org, NCBI)
Gene Name
SLC25A6
Full Name
ADP/ATP translocase 3
Weight
32866 MW
Superfamily
mitochondrial carrier (TC 2.A.29) family
Alternative Names
ADP/ATP translocase 3;ADP,ATP carrier protein 3;ADP,ATP carrier protein, isoform T2;ANT 2;Adenine nucleotide translocator 3;ANT 3;Solute carrier family 25 member 6;ADP/ATP translocase 3, N-terminally processed;SLC25A6;ANT3;CDABP0051; SLC25A6 AAC3, ANT, ANT 2, ANT 3, ANT3, ANT3Y solute carrier family 25 member 6 ADP/ATP translocase 3|ADP,ATP carrier protein 3|ADP,ATP carrier protein, liver|ADP/ATP translocator of liver|adenine nucleotide translocator 3|epididymis secretory sperm binding protein|solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC25A6, check out the SLC25A6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC25A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADP/ATP translocase 3 SLC25A6 Antibody (A09457)
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No publications found for A09457
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Customer Q&As
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7 Customer Q&As for Anti-ADP/ATP translocase 3 SLC25A6 Antibody
Question
We are currently using anti-SLC25A6 antibody A09457 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The anti-SLC25A6 antibody (A09457) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-10
Question
I was wanting to use your anti-SLC25A6 antibody for WB for human thyroid gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thyroid gland identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
You can see on the product datasheet, A09457 anti-SLC25A6 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
Will A09457 anti-SLC25A6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
As indicated on the product datasheet, A09457 anti-SLC25A6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-18
Question
Can you help my question with product A09457, anti-SLC25A6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09457 anti-SLC25A6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-SLC25A6 antibody A09457. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-14
Question
I would like to test anti-SLC25A6 antibody A09457 on human thyroid gland for research purposes, then I may be interested in using anti-SLC25A6 antibody A09457 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Jha
Verified customer
Asked: 2017-01-25
Answer
The products we sell, including anti-SLC25A6 antibody A09457, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-25
Question
Do you have a BSA free version of anti-SLC25A6 antibody A09457 available?
R. Evans
Verified customer
Asked: 2013-09-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SLC25A6 antibody A09457 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-09-04