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Product Info Summary
| SKU: | M03259-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SLC2A3 Antibody (C-Term)
SKU/Catalog Number
M03259-1
Size
50 µl
Description
Boster Bio Anti-SLC2A3 Antibody (C-Term) (Catalog # M03259-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SLC2A3 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03259-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SLC2A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 432-463 amino acids from human SLC2A3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03259-1 is reactive to SLC2A3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
53924 Da
Background of Glut3
Facilitative glucose transporter. Probably a neuronal glucose transporter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03259-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-SLC2A3 Antibody (C-Term) at 1:2000 dilution
Lane 1: C6 whole cell lysate
Lane 2: SH-SY5Y whole cell lysate
Lane 3: human brain lysate
Lane 4: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2 OS cells stained with M03259-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03259-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For SLC2A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A3
Full Name
Solute carrier family 2, facilitated glucose transporter member 3
Weight
53924 Da
Superfamily
major facilitator superfamily
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 3, Glucose transporter type 3, brain, GLUT-3, SLC2A3, GLUT3 SLC2A3 GLUT3 solute carrier family 2 member 3 solute carrier family 2, facilitated glucose transporter member 3|GLUT-3|glucose transporter type 3, brain|solute carrier family 2 (facilitated glucose transporter), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A3, check out the SLC2A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC2A3 Antibody (C-Term) (M03259-1)
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