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Product Info Summary
| SKU: | A17258-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SLC35A4 Antibody Picoband™
SKU/Catalog Number
A17258-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC35A4 Antibody Picoband™ catalog # A17258-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC35A4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17258-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC35A4 recombinant protein (Position: M1-Q142).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A17258-1 is reactive to Slc35a4 in Human, Mouse, Rat
Applications
A17258-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
34.695kDa
Background of Slc35a4
Predicted to enable pyrimidine nucleotide-sugar transmembrane transporter activity. Involved in positive regulation of translation in response to stress. Predicted to be located in Golgi apparatus. Predicted to be integral component of Golgi membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC35A4 using anti-SLC35A4 antibody (A17258-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35A4 antigen affinity purified polyclonal antibody (Catalog # A17258-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35A4 at approximately 36 kDa. The expected band size for SLC35A4 is at 35 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Slc35a4 (Source: Uniprot.org, NCBI)
Gene Name
Slc35a4
Full Name
Probable UDP-sugar transporter protein SLC35A4
Weight
34.695kDa
Superfamily
nucleotide-sugar transporter family
Alternative Names
MGC2541; probable UDP-sugar transporter protein SLC35A4; solute carrier family 35 (UDP-galactose transporter), member A4; Solute carrier family 35 member A4; solute carrier family 35, member A4; tumor rejection antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Slc35a4, check out the Slc35a4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Slc35a4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC35A4 Antibody Picoband™ (A17258-1)
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No publications found for A17258-1
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Customer Reviews
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