Anti-SLC35D1 Antibody Picoband™

Product Name

Anti-SLC35D1 Antibody Picoband™

View all SLC35D1 Antibodies

SKU/Catalog Number

A11935-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC35D1 Antibody Picoband™ catalog # A11935-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SLC35D1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11935-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLC35D1 recombinant protein (Position: M1-V355).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11935-2 is reactive to SLC35D1 in Human, Mouse

Applications

A11935-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

39.24kDa

Background of SLC35D1

Glycosylation of cellular glycoconjugates occurs in the endoplasmic reticulum (ER) and Golgi compartment, and requires transport of nucleotide sugars from the cytosol into the lumen of the ER and Golgi by specific transporters. The protein encoded by this gene resides in the ER, and transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm to the ER lumen. It may participate in glucuronidation and/or chondroitin sulfate biosynthesis. Mutations in this gene are associated with Schneckenbecken dysplasia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SLC35D1 using anti-SLC35D1 antibody (A11935-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35D1 antigen affinity purified polyclonal antibody (Catalog # A11935-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35D1 at approximately 39 kDa. The expected band size for SLC35D1 is at 39 kDa.

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Figure 2. Flow Cytometry analysis of U87 cells using anti-SLC35D1 antibody (A11935-2).
Overlay histogram showing U87 cells stained with A11935-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC35D1 Antibody (A11935-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SLC35D1 (Source: Uniprot.org, NCBI)

Alternative Names

UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter SLC35D1 SHNKND, UGTREL7 solute carrier family 35 member D1 UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter|UDP-GlcA/UDP-GalNAc transporter|UDP-galactose transporter-related 7|UDP-galactose transporter-related protein 7|solute carrier family 35 (UDP-GlcA/UDP-GalNAc transporter), member D1|solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1

For more info on SLC35D1, check out the SLC35D1 Infographic

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In this infographic, you will see the following information for SLC35D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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