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SLC3A1 antibody (A02654-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of U20S cells using anti-SLC3A1 antibody (A02654-2).
Overlay histogram showing U20S cells stained with A02654-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC3A1 Antibody (A02654-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U20S cells stained with A02654-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC3A1 Antibody (A02654-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SLC3A1 Antibody Picoband®
SLC3A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
SLC3A1 antibody was positively detected by WB in:
monkey COS-7 whole cell, human Caco-2 whole cell, rat NRK whole cell, mouse HBZY whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC3A1 antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Monkey
Tested
Recommend
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-SLC3A1 Antibody Picoband®
View all SLC3A1 Antibodies
View all SLC3A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC3A1 recombinant protein (Position: K70-R665).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
110 kDa
Calculated Molecular Weight
78.9 kDa
Background of SLC3A1
Neutral and basic amino acid transport protein rBAT is a protein that in humans is encoded by the SLC3A1 gene. This gene encodes a type II membrane glycoprotein which is one of the components of the renal amino acid transporter which transports neutral and basic amino acids in the renal tubule and intestinal tract. Mutations and deletions in this gene are associated with cystinuria. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Cite This Product
Anti-SLC3A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02654-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Rabbit
87%
Moderate Homology
Cow
84%
Moderate Homology
Dog
84%
Moderate Homology
Sheep
84%
Moderate Homology
Mouse
83%
Moderate Homology
Rat
82%
Moderate Homology
Zebrafish
55%
Low homology
Drosophila
37%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC3A1 antibody
Flow Cytometry (FC) protocol for SLC3A1 antibody
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Product Promise
We promise Anti-SLC3A1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02654-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026