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Product Info Summary
| SKU: | M01016-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SLC7A5 Antibody (Monoclonal, 31S64)
SKU/Catalog Number
M01016-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SLC7A5 Antibody (Monoclonal, 31S64) catalog # M01016-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC7A5 Antibody (Monoclonal, 31S64) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01016-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31S64
Immunogen
Recombinant protein within human SLC7A5 aa 73-505.
Reactive Species
M01016-2 is reactive to SLC7A5 in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
55.0 kDa
Background of SLC7A5
Solute carrier family 7 member 5, also known as Large neutral amino acids transporter small subunit 1 or SLC7A5 is a protein that in humans is encodes by the SLC7A5 gene. This gene is mapped to 16q24.2. This gene acts as an amino acid exchanger. It involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine(T3) and thyroxine(T4) across the cell membrane in tissues such as placenta. This gene plays a role in neuronal cell proliferation(neurogenesis) in brain. It involved in the uptake of methylmercury(MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01016-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
ImmunoPrecipitation, 1:50
Positive Control
WB: human MCF-7 whole cell lysates, human SiHa whole cell
IHC: human colon cancer tissue, human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of SLC7A5 using anti-SLC7A5 antibody (M01016-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A5 antigen affinity purified monoclonal antibody (M01016-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC7A5 at approximately 38 kDa. The expected band size for SLC7A5 is at 55 kDa.
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IHC analysis of SLC7A5 using anti-SLC7A5 antibody (M01016-2).
SLC7A5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SLC7A5 Antibody (M01016-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of SLC7A5 using anti-SLC7A5 antibody (M01016-2).
SLC7A5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SLC7A5 Antibody (M01016-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-SLC7A5 Antibody (Monoclonal, 31S64) (M01016-2)
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