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- Table of Contents
SLIT3 antibody (A04745) performs well in ELISA, WB and shows reactivity with Bovine, Canine, Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot using Boster's Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu (611-432-122) for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Anti-SLIT-3 Antibody
SLIT3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Bovine, Canine, Human, Mouse, Rat | Rabbit | ELISA, WB |
Recommend Dilution
Application
Dilution
Species
ELISA: 1:2
000 - 1:10
000
WB: 1:500 - 1:2
000
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract.
Product Information
Product Name
Anti-SLIT-3 Antibody
View all SLIT3 Antibodies
View all SLIT3 Antibodies
Form
Liquid (sterile filtered)
Uniprot
Gene ID
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.
Cross-Reactivity
No cross reactivity with other proteins.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
167.7 kDa
Background of SLIT3
SLIT-3 (also known as multiple epidermal growth factor-like domain 5 and Slit homolog 3 protein) is a Slit protein. The 'slit' gene has been shown to play a critical role in central nervous system midline formation. In addition to SLIT3 there are two additional human 'slit' homologs, which are termed SLIT1 and SLIT2. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. SLIT proteins may also participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Slit-3 is a secreted protein predominantly expressed in thyroid. Multiple isoforms have been reported for this product.
Cite This Product
Anti-SLIT-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04745)
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Product Promise
We promise Anti-SLIT-3 Antibody performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A04745
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$641.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026