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Product Info Summary
| SKU: | M01627 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Slit2 Monoclonal Antibody
SKU/Catalog Number
M01627
BM5312 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Slit2 Monoclonal Antibody catalog # M01627. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Slit2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01627)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACGF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Slit2 Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors.
Reactive Species
M01627 is reactive to SLIT2 in Human, Mouse, Rat
Observed Molecular Weight
58 kDa
Calculated molecular weight
169.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01627 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of Slit2 expression in SH-SY5Y cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Specific Publications For Anti-Slit2 Monoclonal Antibody (M01627)
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Customer Q&As
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3 Customer Q&As for Anti-Slit2 Monoclonal Antibody
Question
We are currently using anti-Slit2 Monoclonal antibody M01627 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
K. Krishna
Verified customer
Asked: 2020-01-22
Answer
The anti-Slit2 Monoclonal antibody (M01627) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
Will anti-Slit2 Monoclonal antibody M01627 work on pig WB with fetal brain fetal kidney?
C. Collins
Verified customer
Asked: 2017-03-01
Answer
Our lab technicians have not validated anti-Slit2 Monoclonal antibody M01627 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Slit2 Monoclonal antibody M01627 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig fetal brain fetal kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-01
Question
I was wanting to use your anti-Slit2 Monoclonal antibody for IP for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
T. Yang
Verified customer
Asked: 2013-07-23
Answer
It shows on the product datasheet, M01627 anti-Slit2 Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-23
