Anti-SMARCAD1 Antibody Picoband™

SMARCAD1 antibody

Boster Bio Anti-SMARCAD1 Antibody Picoband™ catalog # A06049-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A06049-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-SMARCAD1 Antibody Picoband™

View all SMARCAD1 Antibodies

SKU/Catalog Number

A06049-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMARCAD1 Antibody Picoband™ catalog # A06049-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMARCAD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06049-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SMARCAD1 recombinant protein (Position: D827-T1002).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06049-1 is reactive to SMARCAD1 in Human, Mouse, Rat

Applications

A06049-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of SMARCAD1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 is a protein that in humans is encoded by the SMARCAD1 gene. It is mapped to 4q22.3. This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SMARCAD1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9H4L7

Gene ID

56916

Gene Name

SMARCAD1

Full Name

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

Weight

117.402kDa

Superfamily

SNF2/RAD54 helicase family

Alternative Names

ATP-dependent helicase 1; DKFZP762K2015; EC 3.6.1; EC 3.6.4.12; HEL1; hHEL1; KIAA1122ETL1DKFZp762K2015; subfamily a, containing DEAD/H box 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1; SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin SMARCAD1 ADERM, BASNS, ETL1, HEL1, HRZ SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1|ATP-dependent helicase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SMARCAD1, check out the SMARCAD1 Infographic

SMARCAD1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMARCAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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6 Customer Q&As for Anti-SMARCAD1 Antibody Picoband™

Question

Will A06049-1 anti-SMARCAD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Jones

Verified customer

Asked: 2019-12-13

Answer

You can see on the product datasheet, A06049-1 anti-SMARCAD1 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-13

Question

Will anti-SMARCAD1 antibody A06049-1 work on pig IHC-P with liver?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

Our lab technicians have not tested anti-SMARCAD1 antibody A06049-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SMARCAD1 antibody A06049-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-17

Question

I see that the anti-SMARCAD1 antibody A06049-1 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-04

Question

We are currently using anti-SMARCAD1 antibody A06049-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-22

Answer

The anti-SMARCAD1 antibody (A06049-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-22

Question

Do you have a BSA free version of anti-SMARCAD1 antibody A06049-1 available?

D. Rodriguez

Verified customer

Asked: 2018-09-12

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SMARCAD1 antibody A06049-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-09-12

Question

Can you help my question with product A06049-1, anti-SMARCAD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Patel

Verified customer

Asked: 2013-11-01

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06049-1 anti-SMARCAD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-11-01

Size

Conjugation

Total: $315

SKU:A06049-1

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A06049-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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