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- Table of Contents
SMCHD1 antibody (A04751-1) performs well in ELISA, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of SMCHD1 and Tubulin beta using anti-SMCHD1 antibody (A04514-2) and anti-Tubulin beta antibody (M05613-4).
SMCHD1 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMCHD1 Antibody (A04514-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SMCHD1 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMCHD1 Antibody (A04514-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-SMCHD1 Antibody Picoband®
SMCHD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
SMCHD1 antibody was positively detected by WB in:
human HepG2 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SMCHD1 antibody was positively detected by ICC/IF in:
U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Sheep
Predicted
Predicted
Bovine
Predicted
Predicted
Product Information
Product Name
Anti-SMCHD1 Antibody Picoband®
View all SMCHD1 Antibodies
View all SMCHD1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SMCHD1 recombinant protein (Position: Q655-D1765).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
226 kDa
Calculated Molecular Weight
226.4 kDa
Background of SMCHD1
Structural Maintenance of Chromosomes flexible Hinge Domain Containing 1 (SMCHD1) is a protein that in humans is encoded by the SMCHD1 gene. This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family.
Cite This Product
Anti-SMCHD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04751-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
93%
High Homology
Rabbit
93%
High Homology
Sheep
92%
High Homology
Cow
92%
High Homology
Rat
87%
Moderate Homology
Mouse
85%
Moderate Homology
Zebrafish
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SMCHD1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SMCHD1 antibody
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Product Promise
We promise Anti-SMCHD1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A04751-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026