Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody

Myosin heavy chain 11 antibody

Boster Bio Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody (Catalog # M02422). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Cow, Pig, Dog, Cat, Rabbit, Rat, Guinea pig, Chicken. Cited in 4 publication(s).

Product Info Summary

SKU: M02422
Size: 100ug/vial
Reactive Species: Chicken, Cow, Dog, Guinea pig, Human, Pig, Rabbit, Rat, Cat
Host: Mouse
Application: Flow Cytometry, IF, IHC

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Product Name

Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody

View all Myosin heavy chain 11 Antibodies

SKU/Catalog Number

M02422

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody (Catalog # M02422). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Cow, Pig, Dog, Cat, Rabbit, Rat, Guinea pig, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02422)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ID8

Isotype

IgG1, kappa

Immunogen

Human uterus extract

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02422 is reactive to MYH11 in Chicken, Cow, Dog, Guinea pig, Human, Pig, Rabbit, Rat, Cat

Applications

M02422 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

227.339kDa

Background of Myosin heavy chain 11

Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MYH11 (Source: Uniprot.org, NCBI)

Gene Name

MYH11

Full Name

Myosin-11

Weight

227.339kDa

Superfamily

TRAFAC class myosin-kinesin ATPase superfamily

Alternative Names

AAT4; FAA4; MYH11 myosin, heavy chain 11, smooth muscle; MYH11; SMHC; SMMHC; Smooth muscle myosin heavy chain MYH11 AAT4, FAA4, SMHC, SMMHC myosin heavy chain 11 myosin-11|epididymis secretory sperm binding protein|myosin, heavy chain 11, smooth muscle|myosin, heavy polypeptide 11, smooth muscle

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MYH11, check out the MYH11 Infographic

MYH11 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYH11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02422 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The penetration and phenotype modulation of smooth muscle cells on surface heparin modified poly(ɛ‐caprolactone) vascular scaffold

All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells\n

All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells\n

Cao, J., Geng, X., Wen, J., Li, Q., Ye, L., Zhang, A.,…, & Gu, Y. (2017). The penetration and phenotype modulation of smooth muscle cells on surface heparin modified poly(ɛ-caprolactone) vascular scaffold. Journal of Biomedical Materials Research....

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7 Customer Q&As for Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody

Question

Please see the WB image, lot number and protocol we used for hippocampus using anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-16

Question

I see that the anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-19

Question

Would anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 work on pig IHC with liver?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

Our lab technicians have not tested anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-10

Question

I was wanting to use your anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody for IHC for guinea pig hippocampus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for guinea pig hippocampus identification?

Verified Customer

Verified customer

Asked: 2019-05-06

Answer

As indicated on the product datasheet, M02422 anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody has been validated for IHC on bovine, canine, chicken, guinea pig, human, pig, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in guinea pig hippocampus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-06

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus using anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-03-04

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-04

Question

Will anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 work for IHC with hippocampus?

P. Carter

Verified customer

Asked: 2018-05-29

Answer

According to the expression profile of hippocampus, MYH11 is highly expressed in hippocampus. So, it is likely that anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 will work for IHC with hippocampus.

Boster Scientific Support

Answered: 2018-05-29

Question

Will M02422 anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

As indicated on the product datasheet, M02422 anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-24

Order DetailsPrice
M02422-100ug

100 μg

$468

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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