Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4)

alpha-Smooth Muscle Actin antibody

Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) catalog # MA1106. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1106
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IF, IHC, IHC-F, WB

Product Name

Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4)

View all alpha-Smooth Muscle Actin Antibodies

SKU/Catalog Number

MA1106

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) catalog # MA1106. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1106)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

1A4

Isotype

Mouse IgG2a

Immunogen

N-terminal synthetic decapeptide of alpha-smooth muscle actin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1106 is reactive to ACTA2 in Human, Mouse, Rat

Applications

MA1106 is guaranteed for IF, IHC, IHC-F, WB Boster Guarantee

Background of alpha-Smooth Muscle Actin

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 2μg/ml, Human, mouse, rat
Immunohistochemistry (Paraffin-embedded Section), 0.1-0.5μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 0.1-0.5μg/ml, Human, mouse, rat,
Immunofluorescence, 1 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACTA2 (Source: Uniprot.org, NCBI)

Uniprot ID

P62738

Gene ID

81633

Gene Name

ACTA2

Full Name

Actin, aortic smooth muscle

Weight

42.009kDa

Superfamily

actin family

Alternative Names

1a4 sma; 1a4 smooth muscle actin; AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha sma 1a4; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; sma 1a4; SMA; smooth muscle actin Acta2|actin alpha 2, smooth muscle|actin, aortic smooth muscle|actin, alpha 2, smooth muscle, aorta|alpha-actin-2|smooth muscle alpha-actin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTA2, check out the ACTA2 Infographic

ACTA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1106 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

EPHB4 Regulates Human Trophoblast Cell Line HTR-8/SVneo Function: Implications for the Role of EPHB4 in Preeclampsia

Fang L,Wang KK,Zhang PF,Li T,Xiao ZL,Yang M,Yu ZX.Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF-BB.J Cell Mol Med.2020 Jan;24(2) :1917-1933.doi:10.1111/jcmm.14888.Epub 2020 Jan 1.PMI
Species: Rat
MA1106 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA

Shen J,Wei W,Wang X,Yang J,Lu L,Lv X,Xue X.Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation.Evid Based Complement Alternat Med.2020 Aug 22;2020:72
Species: Rat
MA1106 usage in article: APP:IHC, SAMPLE:VSMCS, DILUTION:1:1000

Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
MA1106 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000

Liao J, Wu Q, Qian C, Zhao N, Zhao Z, Lu K, Zhang S, Dong Q, Chen L, Li Q, Du Y. TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats. JCI Insight. 2020 Oct 29:137528. doi: 10.1172/jci.insight.137528. Epub ahead of print. PMID: 33119
Species: Rat
MA1106 usage in article: APP:IHC, SAMPLA:ATRIA TISSUE, DILUTION:1:50

Yang J, Li J, Tan R, He X, Lin X, Zhong X, Fan J, Wang L. Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation. Phytother Res. 2020 Oct 28. doi:10.1002/ptr.6 919. Epub ahead of print. PMID: 33118280.
Species: Mouse
MA1106 usage in article: APP:WB, SAMPLE:MOUSE KIDNEY TISSUES, DILUTION:1:1000

Decreased endogenous hydrogen sulfide generation in penile tissues of diabetic rats with erectile dysfunction

Phenotypic switching of vascular smooth muscle cells in animal model of rat thoracic aortic aneurysm

Suppressive effects of 17%u03B2-estradiol on hepatic fibrosis in CCl4-induced rat model

Activation of AMPK by simvastatin inhibited breast tumor angiogenesis via impeding HIF%u20101%u03B1%u2010induced pro%u2010angiogenic factor

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1 Customer Q&As for Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4)

Question

Our lab want to know about using your anti-Alpha-Smooth Muscle Actin antibody (Monoclonal, 1A4) for r-rno-445355; smooth muscle contraction studies. Has this antibody been tested with western blotting on rat stomach tissue? We would like to see some validation images before ordering.

B. Miller

Verified customer

Asked: 2015-02-02

Answer

Thanks for your inquiry. This MA1106 anti-Alpha-Smooth Muscle Actin antibody (Monoclonal, 1A4) is tested on rat stomach tissue, mouse stomach tissue lysates, human placenta tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-02-02

Size

Conjugation

Total: $315

SKU:MA1106

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MA1106
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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