Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4)

Mouse IgG monoclonal antibody for alpha-Smooth Muscle Actin, actin, alpha 2, smooth muscle, aorta (ACTA2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

SKU MA1106
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number 1A4
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC, WB


Product Name Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4)
See all ACTA2 primary antibodies, ELISA kits and proteins
SKU/Catalog Number MA1106
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Monoclonal clone# G2 antibody for 1867 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. 1867 information: Molecular Weight: 42009 MW; Subcellular Localization: Cytoplasm, cytoskeleton.
Cite This Product Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1106)
Specificity Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4) (MA1106) reacts with Human, Mouse, Rat ACTA2, in native form and recombinant. Superfamily members of ACTA2 are not reactive to MA1106.
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunogen N-terminal synthetic decapeptide of alpha-smooth muscle actin.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4) covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.1-0.5μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 0.1-0.5μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4) (MA1106).

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions


Figure 1. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106).
Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1072) with DAB as the chromogen.


Figure 2. Western blot analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: mouse stomach tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Smooth Muscle Actin antigen affinity purified monoclonal antibody (Catalog # MA1106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha-Smooth Muscle Actin at approximately 43KD. The expected band size for Alpha-Smooth Muscle Actin is at 43KD.

Target Info

Protein Target Info (Source:

Uniprot Id P62738
Gene Name ACTA2
Protein Name Actin, aortic smooth muscle
Alternative Names Actin, aortic smooth muscle;Alpha-actin-2;Acta2;Actsa, Actvs;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 42009 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.


Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)



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Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
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