SKU MA1106
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number 1A4
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC, WB

Overview

Product Name Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4)
SKU/Catalog Number MA1106
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for alpha-Smooth Muscle Actin, actin, alpha 2, smooth muscle, aorta (ACTA2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-Alpha-Smooth Muscle Actin Antibody (Monoclonal, 1A4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1106)
Host Mouse
Immunogen N-terminal synthetic decapeptide of alpha-smooth muscle actin.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.1-0.5μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 0.1-0.5μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1106 1 IHC anti alpha smooth muscle actin antibody monoclonal 1a4.jpg

Figure 1. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106).
Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1072) with DAB as the chromogen.

/m/a/ma1106 2.jpg

Figure 2. Western blot analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: mouse stomach tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Smooth Muscle Actin antigen affinity purified monoclonal antibody (Catalog # MA1106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha-Smooth Muscle Actin at approximately 43KD. The expected band size for Alpha-Smooth Muscle Actin is at 43KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P62738
Gene Name ACTA2
Protein Name Actin, aortic smooth muscle
Alternative Names Actin, aortic smooth muscle;Alpha-actin-2;Acta2;Actsa, Actvs;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 42009 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Background Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

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Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
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Troubleshooting

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Publications

The coordinated roles of miR-26a and miR-30c in regulating TGF?1-induced epithelial-to-mesenchymal transition in diabetic nephropathy
Thalidomide Inhibits TGF-?1-induced Epithelial to Mesenchymal Transition in Alveolar Epithelial Cells via Smad-Dependent and Smad-Independent Signaling Pathways
Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-?1
Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-?B and TGF-?1/Smad-2/-3 pathways
Inhibitory effects of oxymatrine on TGF-?1-induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2 signaling pathways
Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-?1/Smad pathway
Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-?1-Induced Epithelial-to-Mesenchymal Transition
Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis
Adenovirus-mediated knockdown of activin A receptor type 2A attenuates immune-induced hepatic fibrosis in mice and inhibits interleukin-17-induced activation of primary hepatic stellate cells
Activation of AMPK inhibits TGF-?1-induced airway smooth muscle cells proliferation and its potential mechanisms
Acetyl-11-Keto-?-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-?1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats
Autophagic degradation of caveolin-1 promotes liver sinusoidal endothelial cells defenestration
Specific Inhibitor of Smad3 (SIS3) Attenuates Fibrosis, Apoptosis, and Inflammation in Unilateral Ureteral Obstruction Kidneys by Inhibition of Transforming Growth Factor ? (TGF-?)/Smad3 Signaling
miR-185 Inhibits Fibrogenic Activation of Hepatic Stellate Cells and Prevents Liver Fibrosis
Interleukin-10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21
Extracorporeal shock wave therapy with low-energy flux density inhibits hypertrophic scar formation in an animal model
The Role and Mechanism of ?-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells
FOXA2 alleviates CCl4-induced liver fibrosis by protecting hepatocytes in mice
Poly(ethylene glycol)-modified silk fibroin membrane as a carrier for limbal epithelial stem cell transplantation in a rabbit LSCD model
Hesperetin alleviates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro
Impact of chronic methamphetamine treatment on the atherosclerosis formation in ApoE?/? mice fed a high cholesterol diet
SIRT3 attenuates AngII-induced cardiac fibrosis by inhibiting myofibroblasts transdifferentiation via STAT3-NFATc2 pathway
Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration
The rhythmic expression of clock genes attenuated in human plaque-derived vascular smooth muscle cells
Rutin suppresses high glucose-induced ACTA2 and p38 protein expression in diabetic nephropathy
BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Evaluation of pharmacological relaxation effect of the natural product naringin on in vitro cultured airway smooth muscle cells and in vivo ovalbumin-induced asthma Balb/c mice
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase
Hypoxia drives the transition of human dermal fibroblasts to a myofibroblast-like phenotype via the TGF-?1/Smad3 pathway
Hepatic SATB1 induces paracrine activation of hepatic stellate cells and is upregulated by HBx
Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1
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Inhibitory effects of dimethyl ?-ketoglutarate in hepatic stellate cell activation
IL-9 Inhibits Viral Replication in Coxsackievirus B3-Induced Myocarditis
TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21
The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-?/Smad signaling and activation of the Nrf2/HO-1 pathway
Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth MuscleCells
Signal Transducer and Activator of Transcription 3/MicroRNA-21 Feedback Loop Contributes to Atrial Fibrillation by Promoting Atrial Fibrosis in a Rat Sterile Pericarditis Model
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Preventive Effects of Rhodiola rosea L on Bleomycin-Induced Pulmonary Fibrosis in Rats
Imperatorin derivative OW1 inhibits the upregulation of TGF-? and MMP-2 in renovascular hypertension-induced cardiac remodeling
Astragalus membranaceusameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transitionin vivoandin vitro
Dietary Flavonoid Hyperoside Induces Apoptosis of Activated Human LX-2 Hepatic Stellate Cell by Suppressing Canonical NF-?B Signaling
Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways
The Endothelial Prolyl-4-Hydroxylase Domain 2/Hypoxia-Inducible Factor 2 Axis Regulates Pulmonary Artery Pressure in Mice
Blockade of Orai1 Store-Operated Calcium Entry Protects against Renal Fibrosis
Effect of Huai Qi Huang on Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells through miR-200a
BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation
Digoxin reduces atherosclerosis in apolipoprotein E-deficient mice
Polarization-sensitive optical projection tomography for muscle fiber imaging
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
Identification of cardiac-related circulating microRNA profile in human chronic heart failure
Effect of P2X4R on airway inflammation and airway remodeling in allergic airway challenge in mice
Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing.
Suppression of the increasing level of acetylcholine-stimulated intracellular Ca2+ in guinea pig airway smooth muscle cells by mabuterol
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
Plumbagin Ameliorates CCl4-Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway
Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-?/Smad signaling in paraquat-induced pulmonary fibrosis
Modulation of fatty acid metabolism is involved in the alleviation of isoproterenol-induced rat heart failure by fenofibrate.
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro
Tissue Transglutaminase-Regulated Transformed Growth Factor-?1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis
An HNF1?-regulated feedback circuit modulates hepatic fibrogenesis via the crosstalk between hepatocytes and hepatic stellate cells
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3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats
Dynamic Contrast-Enhanced Magnetic Resonance Imaging with Gd-EOB-DTPA for the Evaluation of Liver Fibrosis Induced by Carbon Tetrachloride in Rats
Robo1/2 regulate follicle atresia through manipulating granulosa cell apoptosis in mice
Vascular normalization induced by sinomenine hydrochloride results in suppressed mammary tumor growth and metastasis.
CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages
Increased adenosine levels contribute to ischemic kidney fibrosis in the unilateral ureteral obstruction model
Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice
Mig-6Gene Knockout Induces Neointimal Hyperplasia in the Vascular Smooth Muscle Cell
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Rho-Associated Protein Kinases Play an Important Role in the Differentiation of Rat Adipose-Derived Stromal Cells into Cardiomyocytes In Vitro
Effects of hydroxysafflor yellow A on proliferation and collagen synthesis of rat vascular adventitial fibroblasts induced by angiotensin II
Sodium ferulate lowers portal pressure in rats with secondary biliary cirrhosis through the RhoA/Rho-kinase signaling pathway: A preliminary study
BMP-7 attenuates liver fibrosis via regulation of epidermal growth factor receptor
Involvement of IL-13 and Tissue Transglutaminase in Liver Granuloma and Fibrosis afterSchistosoma japonicumInfection
Clinicopathological and prognostic significance of epithelial mesenchymal transition-related protein expression in intrahepatic cholangiocarcinoma
TRPV4 Channel Inhibits TGF-?1-Induced Proliferation of Hepatic Stellate Cells
Targeted NGF siRNA Delivery Attenuates Sympathetic Nerve Sprouting and Deteriorates Cardiac Dysfunction in Rats with Myocardial Infarction
Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat
The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats
Lentivirus vector-mediated Rho guanine nucleotide dissociation inhibitor 2 overexpression induces beta-2 adrenergic receptor desensitization in airway smooth muscle cells
Sophocarpine attenuates liver fibrosis by inhibiting the TLR4 signaling pathway in rats
Continuous Passages Accelerate the Reprogramming of Mouse Induced Pluripotent Stem Cells
Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice
Thymic Stromal Lymphopoietin Attenuates the Development of Atherosclerosis in ApoE?/? Mice
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C-reactive protein/oxidised low-density lipoprotein/?2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway
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Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues
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Duodenum Clamping Trauma Induces Significant Postoperative Intraperitoneal Adhesions on a Rat Model
Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury
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Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6
Characterization of vascular endothelial progenitor cells from chicken bone marrow
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Role of bone morphogenetic proteins in form-deprivation myopia sclera
Delta-like 1 Serves as a New Target and Contributor to Liver Fibrosis Down-regulated by Mesenchymal Stem Cell Transplantation
Role of LOX-1 and ROS in oxidized low-density lipoprotein induced epithelial-mesenchymal transition of NRK52E
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Haemin-enhanced expression of haem oxygenase-1 stabilizes erythrocyte-induced vulnerable atherosclerotic plaques
Development of an optimized protocol for primary culture of smooth muscle cells from rat thoracic aortas
Regulation of smooth muscle contractility by the epithelium in rat vas deferens: role of ATP-induced release of PGE2
RNA interference targeting the platelet-derived growth factor receptor ? subunit ameliorates experimental hepatic fibrosis in rats
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Gao J, Jiang L, Liang Q, Shi J, Hou D, Tang D, Chen S, Kong D, Wang S. Regen Biomater. 2018 Mar;5(2):105-114. doi: 10.1093/rb/rby003. Epub 2018 Mar 6. The grafts modified by heparinization and catalytic nitric oxide generation used for vascular implantation in rats
Lin L, Li R, Cai M, Huang J, Huang W, Guo Y, Yang L, Yang G, Lan T, Zhu K. Oxid Med Cell Longev. 2018 Mar 18;2018:7808656. doi: 10.1155/2018/7808656. eCollection 2018. Andrographolide Ameliorates Liver Fibrosis in Mice: Involvement of TLR4/NF-κB and TGF-β1/Smad2 Signaling Pathways
Yang F, Luo L, Zhu ZD, Zhou X, Wang Y, Xue J, Zhang J, Cai X, Chen ZL, Ma Q, Chen YF, Wang YJ, Luo YY, Liu P, Zhao L. Front Pharmacol. 2017 Dec 19;8:929. doi: 10.3389/fphar.2017.00929. eCollection 2017. Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-β1/Smad7 Signaling Pathway in Vitro and in Vivo
Li Y, Su R, Xu S, Huang Q, Xu H. Neuropsychiatr Dis Treat. 2017 Sep 27;13:2477-2487. doi: 10.2147/NDT.S145069. eCollection 2017. Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.