Anti-Myosin-11 MYH11 Antibody

Product Name

Anti-Myosin-11 MYH11 Antibody

View all Myosin heavy chain 11 Antibodies

SKU/Catalog Number

CI1028

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Myosin-11 MYH11 Antibody (Catalog# CI1028). Tested in ChIP, ChIP-seq, ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.

Cite This Product

Anti-Myosin-11 MYH11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1028)

Host

Rabbit

Contents

Whole antiserum from rabbit containing 0.05% azide.

Clonality

Polyclonal

Immunogen

This antibody is raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

CI1028 is reactive to MYH11 in Human

Applications

CI1028 is guaranteed for ChIP, ChIP-seq, ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

227.339kDa

Background of Myosin heavy chain 11

MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family which function as major contractile proteins. MYH11 is involved in a pericentric inversion of chromosome 16 (inv (16) (p13q22)) which produces a chimeric transcript consisting of the N terminus of CBFb and the C-terminal portion MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.References citing this antibody (1) Mandoli A, Singh AA, Jansen PWTC, Wierenga ATJ, Riahi H, Franci G, Prange K, Saeed S, Vellenga E, Vermeulen M, Stunnenberg HG and Martens JHA (2013) CBFB–MYH11/RUNX1 together with a compendium of hematopoietic regulators, chromatin modifiers and basal transcription factors occupies self-renewal genes in inv (16) acute myeloid leukemia. Leukemia: 1-9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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ChIP assays were performed using ME-1 cells, Anti-MYH11 polyclonal antibody (Catalog # CI1028) and optimized primer pairs for qPCR. QPCR was performed using primers specific for the genes indicated.

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ChIP was performed with 5 μl of Anti-MYH11 polyclonal antibody (Catalog # CI1028)on sheared chromatin from 1.5 million cells. The IP DNA from 6 ChIP was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

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To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-MYH11 polyclonal antibody (Catalog # CI1028). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.

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Western blot analysis of MYH11 expression in nuclear extracts from ME-1 cells. CBFb was detected using Anti-CBFb antibody, whereas CBFb-MYH11 fusion proteins were detected using Anti-CBFb antibody and MYH11 polyclonal antibody (Catalog # CI1028) at 1/1000 dilution.

Gene/Protein Information For MYH11 (Source: Uniprot.org, NCBI)

Alternative Names

AAT4; FAA4; MYH11 myosin, heavy chain 11, smooth muscle; MYH11; SMHC; SMMHC; Smooth muscle myosin heavy chain MYH11 AAT4, FAA4, SMHC, SMMHC myosin heavy chain 11 myosin-11|epididymis secretory sperm binding protein|myosin, heavy chain 11, smooth muscle|myosin, heavy polypeptide 11, smooth muscle

For more info on MYH11, check out the MYH11 Infographic

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In this infographic, you will see the following information for MYH11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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