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Product Info Summary
| SKU: | A00451-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Smoothened/SMO Antibody Picoband®
SKU/Catalog Number
A00451-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smoothened/SMO Antibody Picoband® catalog # A00451-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Smoothened/SMO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00451-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Smoothened/SMO recombinant protein (Position: N476-Q764).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00451-1 is reactive to SMO in Human, Mouse, Rat
Observed Molecular Weight
86 kDa
Calculated molecular weight
86.4 kDa
Background of SMO
Smoothened is a protein that in humans is encoded by the SMO gene. The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00451-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human K562 whole cell, rat lung tissue, rat stomach tissue, mouse lung tissue, mouse NIH/3T3 whole cell
FCM: JK cell
Validation Images & Assay Conditions
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Western blot analysis of Smoothened/SMO using anti-Smoothened/SMO antibody (A00451-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smoothened/SMO antigen affinity purified polyclonal antibody (Catalog # A00451-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smoothened/SMO at approximately 86 kDa. The expected band size for Smoothened/SMO is at 86 kDa.
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Flow Cytometry analysis of JK cells using anti-Smoothened/SMO antibody (A00451-1).
Overlay histogram showing JK cells stained with A00451-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Smoothened/SMO Antibody Picoband® (A00451-1)
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Customer Reviews
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1 Reviews For Anti-Smoothened/SMO Antibody Picoband®
Anti-Smoothened/SMO Antibody for Flow Cytometry--Yair Fisher, GliXogen Therapeutics, CSO
Excellent
Source: Customer Feedback Submission
| SKU | A00451-1 |
|---|---|
| Application | Flow Cytometry |
| Sample | C3H10T1/2 mouse cell line |
"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."
Yair Fisher
Verified customer
Submitted 2021-11-02
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