Anti-Smoothened/SMO Antibody Picoband™

Product Name

Anti-Smoothened/SMO Antibody Picoband™

View all Smoothened Antibodies

SKU/Catalog Number

A00451-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Smoothened/SMO Antibody Picoband™ catalog # A00451-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Smoothened/SMO Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00451-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Smoothened/SMO recombinant protein (Position: N476-Q764).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00451-1 is reactive to SMO in Human, Mouse, Rat

Applications

A00451-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

86 kDa

Calculated molecular weight

86.397kDa

Background of Smoothened

Smoothened is a protein that in humans is encoded by the SMO gene. The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Smoothened/SMO using anti-Smoothened/SMO antibody (A00451-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smoothened/SMO antigen affinity purified polyclonal antibody (Catalog # A00451-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smoothened/SMO at approximately 86 kDa. The expected band size for Smoothened/SMO is at 86 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of JK cells using anti-Smoothened/SMO antibody (A00451-1).
Overlay histogram showing JK cells stained with A00451-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SMO (Source: Uniprot.org, NCBI)

Alternative Names

bnb; CRJS; FZD11; Gx; Protein Gx; SMO; SMOH; SMOHseven transmembrane helix receptor; smoothened (Drosophila) homolog; smoothened homolog (Drosophila); smoothened homolog; Smoothened SMO CRJS, FZD11, Gx, PHLSH, SMO smoothened, frizzled class receptor smoothened homolog|frizzled family member 11|protein Gx|seven transmembrane helix receptor|smoothened, frizzled family receptor|smoothened, seven transmembrane spanning receptor

For more info on SMO, check out the SMO Infographic

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In this infographic, you will see the following information for SMO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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