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Product Info Summary
| SKU: | A30530 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SMRC2 SMARCC2 Antibody
SKU/Catalog Number
A30530
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SMRC2 SMARCC2 Antibody (Catalog# A30530). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMRC2 SMARCC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30530)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SMRC2. Immunogen sequence location: 361-410
Reactive Species
A30530 is reactive to SMARCC2 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
132.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30530 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using BAF170 Polyclonal Antibody diluted at 1:2000.
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Western Blot analysis of various cells using BAF170 Polyclonal Antibody diluted at 1:2000.
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Western blot analysis of lysates from HUVEC and COLO205 cells, using SMRC2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-SMRC2 SMARCC2 Antibody (A30530)
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Customer Q&As
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5 Customer Q&As for Anti-SMRC2 SMARCC2 Antibody
Question
Will anti-SMRC2 antibody A30530 work on feline WB with brain?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Our lab technicians have not validated anti-SMRC2 antibody A30530 on feline. You can run a BLAST between feline and the immunogen sequence of anti-SMRC2 antibody A30530 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-06
Question
I have attached the WB image, lot number and protocol we used for brain using anti-SMRC2 antibody A30530. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
I was wanting to use to test anti-SMRC2 antibody A30530 on mouse brain for research purposes, then I may be interested in using anti-SMRC2 antibody A30530 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
The products we sell, including anti-SMRC2 antibody A30530, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SMRC2 antibody A30530. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-09
Question
We are currently using anti-SMRC2 antibody A30530 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The anti-SMRC2 antibody (A30530) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-13
