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Product Info Summary
| SKU: | M02585-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-SMURF2 Antibody
SKU/Catalog Number
M02585-1
Size
50 µl
Description
Boster Bio Anti-SMURF2 Antibody (Catalog # M02585-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SMURF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02585-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1826CT865.51.28
Isotype
IgG1,k
Immunogen
This SMURF2 antibody is generated from a mouse immunized with a recombinant protein of human SMURF2.
Reactive Species
M02585-1 is reactive to SMURF2 in Human
Calculated molecular weight
86.2 kDa
Background of SMURF2
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02585-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000-1:4000
Validation Images & Assay Conditions
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All lanes : Anti-SMURF2 Antibody at 1:2000-1:4000 dilution
Lane 1: MDA-MB-231 whole cell lysate
Lane 2: U-2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 86 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-SMURF2 Antibody (M02585-1)
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