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- Table of Contents
SMURF2 Antibodies
AND SMURF2 ELISA kits, SMURF2 Proteins
Many biological assays use antibodies to detect SMURF2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SMURF2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SMURF2 antibodies...
Designing a Western blot for SMURF2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SMURF2 Western Blot GuideDesigning an IHC experiment for SMURF2? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the SMURF2 IHC GuideSMURF2 Infographic by Boster Bio
All SMURF2 Products
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SMURF2 Overview
Facts about E3 ubiquitin-protein ligase SMURF2.
E3 ubiquitin-protein ligase SMURF2 (SMURF2)
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to activate their ubiquitination and proteasome-dependent degradation.
Additionally, interaction with SMAD7 activates autocatalytic degradation, which is avoided by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3.
Gene Information
| Human | |
|---|---|
| Gene Name: | SMURF2 |
| Uniprot: | Q9HAU4 |
| Entrez: | 64750 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
DKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; EC 6.3.2; EC 6.3.2.-; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2
Molecular Weight
Mass (kDA):
86.196 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 17q23.3-q24.1 |
| Sequence: | 17; NC_000017.11 (64542282..64662307, complement) |
Tissue Specificity
Widely expressed.
Subcellular Localizations
Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
Diseases
- Malignant Neoplasms
- Neoplasms
- Fibrosis
- Kidney Diseases
- Cell Transformation, Neoplastic
- Carcinoma
- Kidney Fibrosis
- Cell Invasion
- Neoplasm Metastasis
- Malignant Neoplasm Of Breast
- Degenerative Polyarthritis
- Inflammation
Interacting Proteins
- FBXL15
- RNF11
- SMAD1
- SMAD2
- SMAD3
- SMAD7
- UBB
Pathways
- Localization
- Cell Proliferation
- Pathogenesis
- Cell Migration
- Ossification
- Cell Differentiation
- Endocytosis
- Senescence
- Cell Growth
- Nuclear Export
- Oncogenesis
- Endochondral Ossification
- Proteolysis
PTMs
- Ubiquitination
- Phosphorylation
- Acetylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SMURF2
bosterbio.com/bosterbio-gene-info-cards/SMURF2
References
PMID: 11163210 by Kavsak P., et al. Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation.
PMID: 11016919 by Lin X., et al. Smurf2 Is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling.