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Product Info Summary
| SKU: | M02687 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-SNF2H SMARCA5 Monoclonal Antibody
SKU/Catalog Number
M02687
BM5704 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SNF2H SMARCA5 Monoclonal Antibody catalog # M02687. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNF2H SMARCA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02687)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFDB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SNF2H Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails.
Reactive Species
M02687 is reactive to SMARCA5 in Human, Mouse, Rat
Observed Molecular Weight
122 kDa
Calculated molecular weight
121.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02687 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: MCF7 cell
Validation Images & Assay Conditions
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Western blot analysis of SNF2H expression in MCF7 cell lysate.
Specific Publications For Anti-SNF2H SMARCA5 Monoclonal Antibody (M02687)
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Customer Q&As
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6 Customer Q&As for Anti-SNF2H SMARCA5 Monoclonal Antibody
Question
I have a question about product M02687, anti-SNF2H Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02687 anti-SNF2H Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-11
Question
Is there a BSA free version of anti-SNF2H Monoclonal antibody M02687 available?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SNF2H Monoclonal antibody M02687 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-11
Question
I was wanting to use your anti-SNF2H Monoclonal antibody for IF for mouse embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
As indicated on the product datasheet, M02687 anti-SNF2H Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-SNF2H Monoclonal antibody M02687 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
The anti-SNF2H Monoclonal antibody (M02687) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-14
Question
Does anti-SNF2H Monoclonal antibody M02687 work for IF with embryonic kidney?
T. Johnson
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of embryonic kidney, SMARCA5 is highly expressed in embryonic kidney. So, it is likely that anti-SNF2H Monoclonal antibody M02687 will work for IF with embryonic kidney.
Boster Scientific Support
Answered: 2018-11-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-SNF2H Monoclonal antibody M02687. Let me know if you need anything else.
N. Dhar
Verified customer
Asked: 2015-11-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-24
