Anti-SNF2H SMARCA5 Monoclonal Antibody

Product Name

Anti-SNF2H SMARCA5 Monoclonal Antibody

View all SMARCA5/SNF2H Antibodies

SKU/Catalog Number

M02687

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-SNF2H SMARCA5 Monoclonal Antibody catalog # M02687. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SNF2H SMARCA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02687)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFDB-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SNF2H Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02687 is reactive to SMARCA5 in Human, Mouse, Rat

Applications

M02687 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

250 kDa

Calculated molecular weight

121.905kDa

Background of SMARCA5/SNF2H

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of SNF2H expression in MCF7 cell lysate.

Gene/Protein Information For SMARCA5 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.6.1; EC 3.6.4.-; hISWI; hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; ISWI; SMARCA5; SNF2H; SNF2Hsucrose nonfermenting-like 5; subfamily a, member 5; Sucrose nonfermenting protein 2 homolog; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5; WCRF135 SMARCA5 ISWI, SNF2H, WCRF135, hISWI, hSNF2H SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5|sucrose nonfermenting protein 2 homolog|sucrose nonfermenting-like 5

For more info on SMARCA5, check out the SMARCA5 Infographic

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In this infographic, you will see the following information for SMARCA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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