Anti-SNF5/SMARCB1 Antibody Picoband™

Product Name

Anti-SNF5/SMARCB1 Antibody Picoband™

View all SNF5 Antibodies

SKU/Catalog Number

A00500-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SNF5/SMARCB1 Antibody Picoband™ catalog # A00500-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SNF5/SMARCB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00500-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SNF5/SMARCB1 recombinant protein (Position: E105-D358).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00500-2 is reactive to SMARCB1 in Human, Monkey, Rat

Applications

A00500-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

44 kDa

Calculated molecular weight

44.141kDa

Background of SNF5

SMARCB1(SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1), also known as SNF5, INI1 or MALIGNANT RHABDOID TUMOR SUPPRESSOR, is a protein that in humans is encoded by the SMARCB1 gene. The SMARCB1 gene encodes a subunit of the SWI/SNF ATP-dependent chromatin-remodeling complex. The SMARCB1 gene maps to chromosome 22q11.2(Versteege et al., 1998). Wu et al.(2002) noted that GADD34(PPP1R15A) and SNF5 can coexist in a trimeric complex with chimeric leukemic HRX(MLL) fusion proteins, leading to inhibition of GADD34-mediated apoptosis. By mutation analysis, they showed that the GADD34 region homologous to the HSV-1 ICP34.5 protein was necessary for interaction with SNF5. SNF5 could bind independently with the protein phosphatase-1(PP1) catalytic subunit(PPP1CA) and stimulate its activity in solution and in complex with GADD34. SNF5 and PP1 did not compete for GADD34 binding, but rather formed a stable trimeric complex with GADD34. Wu et al.(2002) proposed that GADD34 mediates growth suppression, at least in part, through its interaction with SNF5. They suggested that SNF5 may function as a regulatory subunit of PP1, either independently or together with GADD34.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Monkey, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SNF5/SMARCB1 using anti-SNF5/SMARCB1 antibody (A00500-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: monkey COS-7 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF5/SMARCB1 antigen affinity purified polyclonal antibody (Catalog # A00500-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF5/SMARCB1 at approximately 44 kDa. The expected band size for SNF5/SMARCB1 is at 44 kDa.

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Figure 2. Flow Cytometry analysis of C6 cells using anti-SNF5/SMARCB1 antibody (A00500-2).
Overlay histogram showing C6 cells stained with A00500-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNF5/SMARCB1 Antibody (A00500-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SMARCB1 (Source: Uniprot.org, NCBI)

Alternative Names

BAF47; BAF47hSNF5; BRG1-associated factor 47; hSNFS; Ini1; INI1RTPS1; Integrase interactor 1 protein; malignant rhabdoid tumor suppressor; RDTSNF5 homolog; Sfh1p; SNF5; SNF5L1; Snr1; subfamily b, member 1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1 SMARCB1 BAF47, CSS3, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1|BRG1-associated factor 47|SNF5 homolog|SWI/SNF-related matrix-associated protein|hSNF5|integrase interactor 1 protein|malignant rhabdoid tumor suppressor|protein phosphatase 1, regulatory subunit 144|sucrose nonfermenting, yeast, homolog-like 1

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