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Product Info Summary
| SKU: | M00499 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GATA4 Monoclonal Antibody
SKU/Catalog Number
M00499
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-GATA4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00499)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 10D8.B10.D4
Isotype
IgG1 kappa
Immunogen
GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
Reactive Species
M00499 is reactive to GATA4 in Human
Calculated molecular weight
44.6 kDa
Background of GATA4
GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00499 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2000 - 1:10,000
WB: 1:500 - 1:1000
Anti-GATA4 antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
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Western Blot of Mouse anti-GATA4 Antibody. Lane 1: CCRF-CEM Lysate. Load: 25 µg per lane. Primary antibody: GATA-4 antibody at 1:500 overnight at 4°C. Secondary antibody: anti-mouse IgG HRP antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 44.5 kDa, 44.5 kDa for GATA4.
Specific Publications For Anti-GATA4 Monoclonal Antibody (M00499)
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5 Customer Q&As for Anti-GATA4 Monoclonal Antibody
Question
My question regarding product M00499, anti-GATA4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00499 anti-GATA4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-10
Question
Would M00499 anti-GATA4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
You can see on the product datasheet, M00499 anti-GATA4 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
Would anti-GATA4 Monoclonal antibody M00499 work on dog WB with heart lung?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Our lab technicians have not validated anti-GATA4 Monoclonal antibody M00499 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GATA4 Monoclonal antibody M00499 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog heart lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
Would anti-GATA4 Monoclonal antibody M00499 work for WB with heart lung?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
According to the expression profile of heart lung, GATA4 is highly expressed in heart lung. So, it is likely that anti-GATA4 Monoclonal antibody M00499 will work for WB with heart lung.
Boster Scientific Support
Answered: 2019-04-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart lung using anti-GATA4 Monoclonal antibody M00499. Let me know if you need anything else.
F. Li
Verified customer
Asked: 2017-05-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-05
