Anti-SnoN SKIL Antibody

Product Name

Anti-SnoN SKIL Antibody

View all SKIL Antibodies

SKU/Catalog Number

A04131-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SnoN SKIL Antibody (Catalog # A04131-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SnoN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SnoN SKIL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04131-1)

Host

Rabbit

Contents

SnoN Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN. The immunogen is located within the first 50 amino acids of SnoN.

Reactive Species

A04131-1 is reactive to SKIL in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

77.0 kDa

Background of SKIL

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04131-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SKIL

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