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Product Info Summary
| SKU: | A04131-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SnoN SKIL Antibody
SKU/Catalog Number
A04131-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SnoN SKIL Antibody (Catalog # A04131-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SnoN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SnoN SKIL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04131-1)
Host
Rabbit
Contents
SnoN Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN. The immunogen is located within the first 50 amino acids of SnoN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
OASL antibody is human, mouse and rat reactive.
Reactive Species
A04131-1 is reactive to SKIL in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
76976 MW
Background of SnoN
TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04131-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 μg/mL.
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Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SKIL (Source: Uniprot.org, NCBI)
Gene Name
SKIL
Full Name
Ski-like protein
Weight
76976 MW
Superfamily
SKI family
Alternative Names
SNO, SnoA, SnoI, SnoN, SNO, Ski-like protein, Ski-related oncogene SKIL SNO, SnoA, SnoI, SnoN SKI like proto-oncogene ski-like protein|SKI-like oncogene|ski-related oncogene snoN
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKIL, check out the SKIL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKIL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SnoN SKIL Antibody (A04131-1)
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