Product Info Summary
SKU: | A03527-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SNRNP70 Antibody Picoband™
SKU/Catalog Number
A03527-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNRNP70 Antibody Picoband™ catalog # A03527-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNRNP70 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03527-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human SNRNP70 recombinant protein (Position: M1-D232). Human SNRNP70 shares 100% amino acid (aa) sequence identity with mouse SNRNP70.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03527-2 is reactive to SNRNP70 in Human, Rat
Applications
A03527-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
51.557kDa
Background of SNRP70
snRNP70 also known as U1 small nuclear ribonucleoprotein 70 kDa is a protein that in humans is encoded by the SNRNP70 gene. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Rat
Immunohistochemistry, 2-5 µg/ml, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP70 antigen affinity purified polyclonal antibody (Catalog # A03527-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP70 at approximately 95 kDa. The expected band size for SNRNP70 is at 52 kDa.
Click image to see more details
Figure 2. IHC analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2).
SNRNP70 was detected in a paraffin-embedded section of rat brain(Cerebral cortex) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP70 Antibody (A03527-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2).
SNRNP70 was detected in a paraffin-embedded section of rat brain(Cerebral cortex) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP70 Antibody (A03527-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2).
SNRNP70 was detected in a paraffin-embedded section of rat brain(Midbrain) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP70 Antibody (A03527-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2).
SNRNP70 was detected in a paraffin-embedded section of rat brain(Midbrain) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP70 Antibody (A03527-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2) and anti-Beta Tubulin antibody (M01857-3).
SNRNP70 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRNP70 Antibody (A03527-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of HepG2 cells using anti-SNRNP70 antibody (A03527-2).
Overlay histogram showing HepG2 cells stained with A03527-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP70 Antibody (A03527-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SNRNP70 (Source: Uniprot.org, NCBI)
Gene Name
SNRNP70
Full Name
U1 small nuclear ribonucleoprotein 70 kDa
Weight
51.557kDa
Alternative Names
RNPU1ZU170K; RPU1U1AP; small nuclear ribonucleoprotein 70kDa (U1); Snp1; snRNP70; SNRP70U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; U1-70Ksmall nuclear ribonucleoprotein 70kDa (RNP antigen); U1AP1; U1RNP SNRNP70 RNPU1Z, RPU1, SNRP70, Snp1, U1-70K, U170K, U1AP, U1RNP small nuclear ribonucleoprotein U1 subunit 70 U1 small nuclear ribonucleoprotein 70 kDa|U1 snRNP 70 kDa|small nuclear ribonucleoprotein 70kDa (U1)|small nuclear ribonucleoprotein, U1 70kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNRNP70, check out the SNRNP70 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNRNP70: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNRNP70 Antibody Picoband™ (A03527-2)
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