Product Info Summary
SKU: | PB9747 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband™
View all SOD3/EC-SOD Antibodies
SKU/Catalog Number
PB9747
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband™ catalog # PB9747. Tested in IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9747)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse SOD3 , different from the related rat sequence by nine amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9747 is reactive to SOD3 in Mouse
Applications
PB9747 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
32 kDa
Calculated molecular weight
25.851kDa
Background of SOD3/EC-SOD
SOD3 (SUPEROXIDE DISMUTASE 3), also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al. (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al. (2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SOD3 using anti-SOD3 antibody (PB9747).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.
Lane 1: Mouse Lung Tissue Lysate,
Lane 2: Mouse Kidney Tissue Lysate,
Lane 3: Mouse Cardiac Muscle Tissue Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD3 antigen affinity purified polyclonal antibody (Catalog # PB9747) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD3 at approximately 32 kDa. The expected band size for SOD3 is at 26 kDa.
Click image to see more details
Figure 2. IHC analysis of SOD3 using anti-SOD3 antibody (PB9747).
SOD3 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SOD3 Antibody (PB9747) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SOD3 (Source: Uniprot.org, NCBI)
Gene Name
SOD3
Full Name
Extracellular superoxide dismutase [Cu-Zn]
Weight
25.851kDa
Superfamily
Cu-Zn superoxide dismutase family
Alternative Names
ECSOD; EC-SOD; EC-SODEC 1.15.1.1; extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; superoxide dismutase 3, extracellular SOD3 EC-SOD superoxide dismutase 3 extracellular superoxide dismutase [Cu-Zn]|superoxide dismutase 3, extracellular|testicular tissue protein Li 175
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOD3, check out the SOD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband™ (PB9747)
Hello CJ!
PB9747 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epigallocatechin%u20103%u2010Gallate Attenuates Oxidative Stress and Inflammation in Obstructive Nephropathy via NF%u2010%u03BAB and Nrf2/HO%u20101 Signalling Pathway Regulation
Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband™
Question
We are currently using anti-Superoxide Dismutase 3/SOD3 antibody PB9747 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-Superoxide Dismutase 3/SOD3 antibody (PB9747) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07