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Product Info Summary
| SKU: | A03551-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, IHC-P-Leica |
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Product info
Product Name
Anti-SOST Antibody (N-term)
SKU/Catalog Number
A03551-1
Size
50 µl
Description
Boster Bio Anti-SOST Antibody (N-term) (Catalog # A03551-1). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SOST Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03551-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.
Reactive Species
A03551-1 is reactive to SOST in Human, Mouse, Rat
Calculated molecular weight
24.0 kDa
Background of SOST
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03551-1 is guaranteed for WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
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Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term). A03551-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilution
Lane 1: mouse lung lysates
Lane 2: human liver lysates
Lane 3: Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SOST Antibody (N-term) at 1:1000 dilution
Lane 1: human kidney lysates
Lane 2: human liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-SOST Antibody (N-term) at 1:2000 dilution + Recombinant human SOST protein lysate
Lysates/proteins at 20 ng per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using A03551-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-SOST Antibody (N-term) (A03551-1)
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