Anti-SPARC Antibody

Product Name

Anti-SPARC Antibody

View all SPARC Antibodies

SKU/Catalog Number

PA1585

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SPARC Antibody catalog # PA1585. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SPARC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1585)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SPARC.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1585 is reactive to SPARC in Human

Applications

PA1585 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

34.632kDa

Background of SPARC

SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-SPARC antibody, PA1585, Western blotting
Lane 1: Recombinant Human SPARC Protein 10ng
Lane 2: Recombinant Human SPARC Protein 5ng
Lane 3: Recombinant Human SPARC Protein 2.5ng

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Anti-SPARC antibody, PA1585, Western blotting
WB: HELA Cell Lysate

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Anti-SPARC antibody, PA1585, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Gene/Protein Information For SPARC (Source: Uniprot.org, NCBI)

Alternative Names

Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC SPARC BM-40, OI17, ON, ONT secreted protein acidic and cysteine rich SPARC|basement-membrane protein 40|secreted protein, acidic, cysteine-rich (osteonectin)

For more info on SPARC, check out the SPARC Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPARC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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