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Product Info Summary
| SKU: | PA2088 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SPARC-like protein 1 SPARCL1 Antibody Picoband®
SKU/Catalog Number
PA2088
BA3069 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPARC-like protein 1 SPARCL1 Antibody catalog # PA2088. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPARC-like protein 1 SPARCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2088)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2088 is reactive to SPARCL1 in Human, Mouse, Rat
Observed Molecular Weight
95 kDa
Calculated molecular weight
75.2 kDa
Background of SPARCL1
SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration (Girard and Springer, 1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2088 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Lung Tissue, Mouse Lung Tissue, PANC Cell, A549 Cell, COLO320 Cell, MCF-7 Cell, HT1080 Cell
IHC: Rat Brain Tissue, Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-SPARCL1 antibody, PA2088, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-SPARCL1 antibody, PA2088, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Mouse Lung Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: HT1080 Cell Lysate
Click image to see more details
Anti-SPARCL1 antibody, PA2088, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Specific Publications For Anti-SPARC-like protein 1 SPARCL1 Antibody Picoband® (PA2088)
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Customer Reviews
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1 Reviews For Anti-SPARC-like protein 1 SPARCL1 Antibody Picoband®
BosterBio Sparcl1 Works--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT
Excellent
Source: Biocompare.com
| SKU | PA2088 |
|---|---|
| Applications | Immunohistochemistry |
| Sample | Mouse astrocytes |
| Detection | Confocal/Fluorescent Scope |
"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."
Ruby C
Verified customer
Submitted 2016-09-13
Customer Q&As
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4 Customer Q&As for Anti-SPARC-like protein 1 SPARCL1 Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tonsil using anti-SPARCL1 antibody PA2088. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
Do you have a BSA free version of anti-SPARCL1 antibody PA2088 available?
R. Huang
Verified customer
Asked: 2018-04-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SPARCL1 antibody PA2088 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-19
Question
Is this PA2088 anti-SPARCL1 antibody reactive to the isotypes of SPARCL1?
N. Krishna
Verified customer
Asked: 2013-05-31
Answer
The immunogen of PA2088 anti-SPARCL1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-05-31
Question
We are currently using anti-SPARCL1 antibody PA2088 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
B. Zhang
Verified customer
Asked: 2013-03-22
Answer
The anti-SPARCL1 antibody (PA2088) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-22
