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Product Info Summary
| SKU: | A18198 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPATA1 Antibody
View all Spermatogensis associated protein 1 Antibodies
SKU/Catalog Number
A18198
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPATA1 Antibody (Catalog # A18198). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SPATA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPATA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18198)
Host
Rabbit
Contents
SPATA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPATA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA1. The immunogen is located within amino acids 300 - 350 of SPATA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of SPATA1 are known to exist; this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.
Reactive Species
A18198 is reactive to SPATA1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
52946 MW
Background of Spermatogensis associated protein 1
SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18198 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPATA1 in A20 cell lysate with SPATA1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SPATA1 in mouse testis tissue with SPATA1 antibody at 2.5 μg/mL.
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Immunofluorescence of SPATA1 in mouse testis tissue with SPATA1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SPATA1 (Source: Uniprot.org, NCBI)
Gene Name
SPATA1
Full Name
Spermatogenesis-associated protein 1
Weight
52946 MW
Alternative Names
SP-2, SPAP1, Spermatogenesis-associated protein 1, Sperm-specific protein SP-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPATA1, check out the SPATA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPATA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPATA1 Antibody (A18198)
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