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Product Info Summary
| SKU: | A14863 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPATA6 Antibody
SKU/Catalog Number
A14863
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPATA6 Antibody (Catalog # A14863). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SPATA6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPATA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14863)
Host
Rabbit
Contents
SPATA6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPATA6 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SPATA6. The immunogen is located within amino acids 400 - 450 of SPATA6.
Cross-reactivity
At least three isoforms of SPATA6 are known to exist; this antibody will detect only the longest two isoforms. SPATA6 antibody is predicted to not cross-react with other SPATA protein family members.
Reactive Species
A14863 is reactive to SPATA6 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
56.0 kDa
Background of SPATA6
The development of spermatogonial stem cells into spermatozoa, i.e., spermatogenesis, is a highly ordered process that requires multiple proteins, including some that are testis-specific or testis-specific isoforms of genes that are expressed in other tissues. One such protein is SPATA6: while its full-length isoform is expressed in multiple tissues including thymus, brain and testis, the shorter isoform is expressed only in testis. It has some homology to the motor domain of kinesin related proteins and with the C. elegans neural calcium sensor protein NCS-2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14863 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Recommend Dilution
SPATA6 antibody can be used for detection of SPATA6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPATA6 in A20 cell lysate with SPATA6 antibody at 1 μg/mL.
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Immunohistochemistry of SPATA6 in mouse testis tissue with SPATA6 antibody at 2.5 μg/mL.
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Immunofluorescence of SPATA6 in mouse testis tissue with SPATA6 antibody at 20 μg/mL.
Specific Publications For Anti-SPATA6 Antibody (A14863)
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