Anti-SPG15 ZFYVE26 Antibody

Product Name

Anti-SPG15 ZFYVE26 Antibody

View all ZFYVE26 Antibodies

SKU/Catalog Number

A06231

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SPG15 ZFYVE26 Antibody (Catalog # A06231). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SPG15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SPG15 ZFYVE26 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06231)

Host

Rabbit

Contents

SPG15 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SPG15 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human SPG15. The immunogen is located within amino acids 1710 - 1760 of SPG15.

Cross-reactivity

Multiple isoforms of SPG15 are known to exist.

Reactive Species

A06231 is reactive to ZFYVE26 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

284.6 kDa

Background of ZFYVE26

Hereditary spastic paraplegias (HSPs) are genetically and phenotypically heterogeneous disorders.  Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration. SPG15 is the second gene known to be responsible for ARHSP-TCC in the Italian population. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. SPG15 encodes a protein containing a FYVE zinc finger binding domain which is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. SPG15 mRNA is widely distributed in human tissues, as well as in rat embryos, suggesting a possible role for this protein during embryonic development. SPG15 co-localizes partially with endoplasmic reticulum and endosome markers, suggesting a role in intracellular trafficking.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06231 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SPG15 antibody can be used for detection of SPG15 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ZFYVE26

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