Anti-Sphingosine kinase 1 SPHK1 Antibody

Product Name

Anti-Sphingosine kinase 1 SPHK1 Antibody

View all SPHK1 Antibodies

SKU/Catalog Number

A01390

Size

100ul

Form

Liquid

Description

SPHK1 (sphingosine kinase 1) catalyzes phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), a lipid mediator with intracellular functions (e.g., proliferation/survival) and extracellular receptor signaling; SPHK1/S1P is described as participating in TNF-α signaling and NF-κB activation relevant to inflammatory/antiapoptotic/immune processes. NCBI Reactome context: SPHK1-mediated conversion of sphingosine to S1P is represented as a defined reaction event in sphingolipid/S1P signaling. Reactome Assay context: rabbit polyclonal IgG, liquid (1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol), immunogen = recombinant full-length human SPHK1; reactive species human/mouse/rat; validated for WB and IHC (paraffin), with starting dilutions WB 1:500–1:2000 and IHC 1:50–1:200; calculated MW 42.5 kDa; IHC validation shown in human stomach cancer tissue (contextual). Bosterbio Often contextualized with immune/tumor microenvironment readouts such as CXCL10 (IP-10) and ectonucleotidase CD73/NT5E (putative), and with drug response/transport markers like ABCB1 (P-gp) (putative).

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Sphingosine kinase 1 SPHK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01390)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Recombinant full length Human SPHK1.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01390 is reactive to SPHK1 in Human, Mouse, Rat

Calculated molecular weight

42.5 kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01390 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
IHC: 1:50-1:200

Validation Images & Assay Conditions

See full target information SPHK1

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