Sphingosine kinase 1 Antibodies

AND SPHK1 ELISA kits, Sphingosine kinase 1 Proteins

Many biological assays use antibodies to detect Sphingosine kinase 1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Sphingosine kinase 1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Sphingosine kinase 1 antibodies...

We validate the specificity of these antibodies to Sphingosine kinase 1 by testing them on tissues known to express SPHK1 positively and negatively. Browse below to find the SPHK1 antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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SPHK1 Infographic by Boster Bio

All SPHK1 Products

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Product

Figures

Specificity

Applications

Price

Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®

6 Q&As

Human

ELISA

$750
Cat # EK1501
96 wells/kit, with removable strips.

Anti-SPHK1 Monoclonal Antibody

1 Q&As

Western blot analysis of SPHK1 expression in Raji cell lysate

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature

Human, Mouse, Rat

Flow Cytometry, WB

$370
Cat # M01390
100 μl

Anti-Sphingosine kinase 1 SPHK1 Antibody

WesternBlot (WB) analysis of SPHK1 polyclonal antibody

Immunohistochemistry (IHC) analysis of SPHK1 pAb in paraffin-embedded Human Stomach Cancer tissue

Human, Mouse, Rat

IHC, WB

$405
Cat # A01390
100ul

Anti-SPHK1 Mouse Monoclonal Antibody [Clone ID: OTI1A6]

Anti-SPHK1 mouse monoclonal antibody (M01390-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPHK1

Immunofluorescent staining of HeLa cells using anti-SPHK1 mouse monoclonal antibody (M01390-1)

Human

IF, IHC, WB

$523
Cat # M01390-1
100 µl

Anti-SPHK1 Antibody Picoband®

3 Citations

Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1)

IHC analysis of SPHK1 using anti-SPHK1 antibody (A01390-1)

+12

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A01390-1
100 μg/vial

SPHK1 (NM_021972) Human Over-expression Lysate

SPHK1 Human Over-expression Lysates NM_021972

Human

$628
Cat # LS008877
20 µg, 100 µg

Anti-SPHK1 Antibody Picoband®

Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-2)

Flow Cytometry analysis of HepG2 cells using anti-SPHK1 antibody (A01390-2)

Human

ELISA, Flow Cytometry, WB

$370
Cat # A01390-2
100 µg/vial

Anti-SPHK1 Antibody (Monoclonal, 33S24)

Human

Flow Cytometry, IHC, WB

$370
Cat # M01390-2
100 μl/vial

SPHK1 Overview

Facts about Sphingosine kinase 1.

SPHK1 3D structure. Source: alphafold

Sphingosine kinase 1 (SPHK1)

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N- dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Gene Information

Human
Gene Name:	SPHK1
Uniprot:	Q9NYA1
Entrez:	8877

Family

Belongs to:

No superfamily

Alternative Names

EC 2.7.1.91; SK1; sphingosine kinase 1; SPHK1; SPHKSK 1; SPK 1; SPK; SPK1

Molecular Weight

Mass (kDA):
42.518 kDA

Genomic Context


Human

Location:	17q25.1
Sequence:	17; NC_000017.11 (76383204..76387855)

Tissue Specificity

Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.

Subcellular Localizations

Cytoplasm. Nucleus. Cell membrane. Endosome membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity).