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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, WB|
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Anti-SPHK1 Monoclonal Antibody
Boster Bio Anti-SPHK1 Monoclonal Antibody catalog # M01390. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPHK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01390)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human SPHK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01390 is reactive to SPHK1 in Human, Mouse, Rat
M01390 is guaranteed for Flow Cytometry, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SPHK1 expression in Raji cell lysate using anti-SPHK1 antibody (M01390).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # M01390) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For SPHK1 (Source: Uniprot.Org, NCBI)
Sphingosine kinase 1
EC 188.8.131.52; SK1; sphingosine kinase 1; SPHK1; SPHKSK 1; SPK 1; SPK; SPK1 SPHK1 SPHK sphingosine kinase 1 sphingosine kinase 1|SK 1|SPK 1|acetyltransferase SPHK1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SPHK1, check out the SPHK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SPHK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M01390 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen C, Gong W, Li C, Xiong F, Wang S, Huang J, Wang Y, Chen Z, Chen Q, Liu P, Lan T, Huang H. Oncotarget. 2017 Aug 10;8(45):78660-78676. doi: 10.18632/oncotarget.20205. eCollection 2017 Oct 3. Sphingosine kinase 1 mediates AGEs-induced fibronecti...
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