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Pack Size:100μg/vial
Validated Species:Human, Mouse
Application:IHC, ICC, WB
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Product Name Anti-SPHK1/Sphingosine Kinase 1 Antibody
SKU/Catalog Number PA1996
Description Rabbit IgG polyclonal antibody for Sphingosine kinase 1(SPHK1) detection. Tested with WB, IHC-P, ICC in Human;Mouse.
Cite This Product Anti-SPHK1/Sphingosine Kinase 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1996)
Replacement Item This antibody may replace the following items: sc-22702|sc-365401|sc-48825|sc-34528|sc-100441 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human SPHK1(117-133aa AASLNHYAGYEQVTNED), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SPHK1
Protein Name Sphingosine kinase 1
Molecular Weight 42518 MW
Protein Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. .
Tissue Specificity Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. .
Subcellular Localization Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.
Uniprot ID Q9NYA1
Alternative Names Sphingosine kinase 1;SK 1;SPK 1;;SPHK1;SPHK, SPK;
Research Areas |signal transduction|signaling pathway|lipid signaling|lipid kinases| signal transduction|g protein signaling|gpcr| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Sphingosine kinase 1

SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Anti-SPHK1/Sphingosine Kinase 1 Antibody Images

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Anti-SPHK1/Sphingosine Kinase 1 Antibody
Anti-SPHK1 antibody, PA1996, Western blotting
Lane 1: RAJI Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: Hut Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Anti-SPHK1/Sphingosine Kinase 1 Antibody
Anti-SPHK1 antibody, PA1996, IHC(P)
IHC(P): Human Placenta Tissue
Anti-SPHK1/Sphingosine Kinase 1 Antibody
Anti-SPHK1 antibody, PA1996, ICC
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to sphingosine kinase 1 antibody, sphk1 antibody