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- Table of Contents
SPHK1 antibody (A01390-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of A431 cells using anti-SPHK1 antibody (A01390-1).
Overlay histogram showing A431 cells stained with A01390-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (A01390-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A01390-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (A01390-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SPHK1 Antibody Picoband®
SPHK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
SPHK1 antibody was positively detected by WB in:
human HepG2 whole cell, human MCF-7 whole cell, human A431 whole cell, human PC-3 whole cell, human Hela whole cell, human U20S whole cell, human Caco-2 whole cell, rat liver tissue, rat kidney tissue, rat RH35 whole cell, mouse liver tissue, mouse kidney tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SPHK1 antibody was positively detected by IHC in:
human hashintoto's thyroditis tissue, human papillary thyroid carcinoma tissue, human placenta tissue, human gall bladder adenosquamous carcinoma tissue, human gastric cancer tissue, human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human lung cancer tissue, rat brain tissue, rat lung tissue, mouse brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SPHK1 antibody was positively detected by ICC/IF in:
HepG2 cell
SPHK1 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SPHK1 Antibody Picoband®
View all SPHK1 Antibodies
View all SPHK1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SPHK1 recombinant protein (Position: R16-H267).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45-50 kDa
Calculated Molecular Weight
42.5 kDa
Background of SPHK1
SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.
Cite This Product
Anti-SPHK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01390-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
90%
High Homology
Dog
90%
High Homology
Mouse
85%
Moderate Homology
Cow
83%
Moderate Homology
Sheep
83%
Moderate Homology
Rat
80%
Moderate Homology
Zebrafish
56%
Low homology
Drosophila
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SPHK1 antibody
Immunohistochemistry (IHC) protocol for SPHK1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SPHK1 antibody
Flow Cytometry (FC) protocol for SPHK1 antibody
Publications
Application:
Species:
The Integration Methodology of Metabolomics and Bioinformatics to Investigate the Mechanism of the Jiawei Erzhi Formula in Treating Postmenopausal Osteoporosis Based on the Kidney-Bone Axis
Published: 2025-08-06
Journal: Separation Science Plus
Application: WB
Impact Factor: 1.60
Species: Rat
Sphingosine kinase 1 promotes M2 macrophage infiltration and enhances glioma cell migration via the JAK2/STAT3 pathway
Published: 2025-02-04
Journal: Scientific Reports
Application: IHC
Impact Factor: 3.90
Species: Human
PMID: 39900970
Polydatin attenuates hepatic stellate cell proliferation and liver fibrosis by suppressing sphingosine kinase 1
Published: 2020-08-17
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IF
Impact Factor: 4.55
Species: Mouse
PMID: 34321171
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Product Promise
We promise Anti-SPHK1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01390-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026