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Product Info Summary
| SKU: | A07281-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
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Product info
Product Name
Anti-SPIN1 antibody
SKU/Catalog Number
A07281-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SPIN1 antibody catalog # A07281-2. Tested in WB, ICC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-SPIN1 antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07281-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human SPIN1 recombinant protein (Position: M1-K260)
Reactive Species
A07281-2 is reactive to SPIN1 in Human, Mouse, Rat
Observed Molecular Weight
30 kDa
Calculated molecular weight
29.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07281-2 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:50
ELISA, 1:100-1000
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human OVCAR-8 whole cell, rat brain tissue, rat C6 tissue, mouse brain tissue
IHC: rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of SPIN1 using anti-SPIN1 antibody (A07281-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human OVCAR-8 whole cell lysates,
Lane 4: human OVCAR-8 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPIN1 antigen affinity purified polyclonal antibody (A07281-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPIN1 at approximately 30 kDa. The expected band size for SPIN1 is at 30 kDa.
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IHC analysis of SPIN1 using anti-SPIN1 antibody (A07281-2).
SPIN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SPIN1 Antibody (A07281-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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