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- Table of Contents
SPIN1 antibody (A07281-2) performs well in ELISA, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of SPIN1 using anti-SPIN1 antibody (A07281-2).
SPIN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SPIN1 Antibody (A07281-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SPIN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SPIN1 Antibody (A07281-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-SPIN1 antibody
SPIN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IF, ICC, WB |
Tested Applications
SPIN1 antibody was positively detected by WB in:
human Hela whole cell, human PC-3 whole cell, human OVCAR-8 whole cell, rat brain tissue, rat C6 tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SPIN1 antibody was positively detected by IHC in:
rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunocytochemistry/Immunofluorescence
1:50-400
Immunoprecipitation
1:50
ELISA
1:100-1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
ELISA
Human
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-SPIN1 antibody
View all SPIN1 Antibodies
View all SPIN1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human SPIN1 recombinant protein (Position: M1-K260)
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
29.6 kDa
Cite This Product
Anti-SPIN1 antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07281-2)
Protocols
Western Blot protocol for SPIN1 antibody
Immunohistochemistry (IHC) protocol for SPIN1 antibody
Review
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Product Promise
We promise Anti-SPIN1 antibody performs excellently in relevant applications for ELISA, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A07281-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026