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Product Info Summary
| SKU: | A09902 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPINSTER SPNS1 Antibody
SKU/Catalog Number
A09902
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPINSTER SPNS1 Antibody (Catalog # A09902). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
SPINSTER antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SPINSTER SPNS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09902)
Host
Rabbit
Contents
SPINSTER antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER. The immunogen is located within the first 50 amino acids of SPINSTER.
Cross-reactivity
SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.
Reactive Species
A09902 is reactive to SPNS1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
56.6 kDa
Background of SPNS1
SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09902 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.
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Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.
Specific Publications For Anti-SPINSTER SPNS1 Antibody (A09902)
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