Product Info Summary
SKU: | A08240-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-Splicing factor 3B subunit 3 SF3B3 Antibody
SKU/Catalog Number
A08240-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Splicing factor 3B subunit 3 SF3B3 Antibody catalog # A08240-2. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Splicing factor 3B subunit 3 SF3B3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08240-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Nap1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08240-2 is reactive to SF3B3 in Human, Mouse, Rat
Applications
A08240-2 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
135.577kDa
Background of SAP130
Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.
Fujita F., Mol. Cell. Biol. 23:7780-7793(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Sasai M., J. Immunol. 174:27-30(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:100
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of SF3B3 using Anti-Splicing factor 3B subunit 3 SF3B3 Antibody (A08240-2).
ICC staining SF3B3 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Splicing factor 3B subunit 3 SF3B3 Antibody A08240-2
Western blot analysis of SF3B3 on Jurkat and Hela cells lysates using anti-SF3B3 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For SF3B3 (Source: Uniprot.org, NCBI)
Gene Name
SF3B3
Full Name
Splicing factor 3B subunit 3
Weight
135.577kDa
Superfamily
RSE1 family
Alternative Names
KIAA0017splicing factor 3b, subunit 3, 130kD; Pre-mRNA-splicing factor SF3b 130 kDa subunit; RSE1; SAP130SAP 130; SF3B130; SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit; Spliceosome-associated protein 130; splicing factor 3B subunit 3; splicing factor 3b, subunit 3, 130kDa; STAF130 SF3B3 RSE1, SAP130, SF3b130, STAF130 splicing factor 3b subunit 3 splicing factor 3B subunit 3|SAP 130|pre-mRNA splicing factor SF3b, 130 kDa subunit|spliceosome-associated protein 130
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SF3B3, check out the SF3B3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SF3B3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Splicing factor 3B subunit 3 SF3B3 Antibody (A08240-2)
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No publications found for A08240-2
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