Anti-Splicing Factor SF3B4 Monoclonal Antibody

Boster Bio Anti-Splicing Factor SF3B4 Monoclonal Antibody catalog # M06414. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M06414
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IF, IHC, WB

Product Name

Anti-Splicing Factor SF3B4 Monoclonal Antibody

See all SF3B4 products

SKU/Catalog Number

M06414

Size

100ul

Description

Boster Bio Anti-Splicing Factor SF3B4 Monoclonal Antibody catalog # M06414. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Splicing Factor SF3B4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06414)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

Clonality

Monoclonal

Immunogen

Full length recombinant human SF3B4 expressed in and purified from E. coli

Reactive Species

M06414 is reactive to SF3B4 in Human, Mouse, Rat

Applications

M06414 is guaranteed for IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:1,000. IF/ICC and IHC: 1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For SF3B4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15427

Gene ID

10262

Gene Name

SF3B4

Full Name

Splicing factor 3B subunit 4

Weight

44.386kDa

Superfamily

SF3B4 family

Alternative Names

Hsh49; pre-mRNA splicing factor SF3b 49 kDa subunit; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49MGC10828; SF3b49; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); Spliceosome-associated protein 49; splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa SF3B4 AFD1, Hsh49, SAP49, SF3b49 splicing factor 3b subunit 4 splicing factor 3B subunit 4|SAP 49|SF3b50|pre-mRNA-splicing factor SF3b 49 kDa subunit|spliceosomal protein|spliceosome-associated protein (U2 snRNP)|spliceosome-associated protein 49|splicing factor 3b, subunit 4, 49kD|splicing factor 3b, subunit 4, 49kDa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SF3B4, check out the SF3B4 Infographic

SF3B4 infographic

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In this infographic you will see the following information for SF3B4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-Splicing Factor SF3B4 Monoclonal Antibody

Question

Would anti-Splicing Factor SF3B4 Monoclonal antibody M06414 work on dog IHC with skin testis?

W. Williams

Verified customer

Asked: 2020-01-22

Answer

Our lab technicians have not validated anti-Splicing Factor SF3B4 Monoclonal antibody M06414 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Splicing Factor SF3B4 Monoclonal antibody M06414 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog skin testis in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-22

Question

I see that the anti-Splicing Factor SF3B4 Monoclonal antibody M06414 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-10

Question

Our lab want to know about to test anti-Splicing Factor SF3B4 Monoclonal antibody M06414 on mouse skin testis for research purposes, then I may be interested in using anti-Splicing Factor SF3B4 Monoclonal antibody M06414 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

The products we sell, including anti-Splicing Factor SF3B4 Monoclonal antibody M06414, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-06

Question

Will M06414 anti-Splicing Factor SF3B4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

It shows on the product datasheet, M06414 anti-Splicing Factor SF3B4 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-15

Question

Would anti-Splicing Factor SF3B4 Monoclonal antibody M06414 work for IHC with skin testis?

Verified Customer

Verified customer

Asked: 2019-02-22

Answer

According to the expression profile of skin testis, SF3B4 is highly expressed in skin testis. So, it is likely that anti-Splicing Factor SF3B4 Monoclonal antibody M06414 will work for IHC with skin testis.

Boster Scientific Support

Answered: 2019-02-22

Question

Is this M06414 anti-Splicing Factor SF3B4 Monoclonal antibody reactive to the isotypes of SF3B4?

K. Anderson

Verified customer

Asked: 2017-05-31

Answer

The immunogen of M06414 anti-Splicing Factor SF3B4 Monoclonal antibody is Full length recombinant human SF3B4 expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-05-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin testis using anti-Splicing Factor SF3B4 Monoclonal antibody M06414. Let me know if you need anything else.

T. Huang

Verified customer

Asked: 2016-03-28

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-03-28

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M06414
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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