Anti-Splicing Factor SF3B4 Monoclonal Antibody

Product Name

Anti-Splicing Factor SF3B4 Monoclonal Antibody

View all SF3B4 Antibodies

SKU/Catalog Number

M06414

Size

100ul

Description

Boster Bio Anti-Splicing Factor SF3B4 Monoclonal Antibody catalog # M06414. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Splicing Factor SF3B4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06414)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Monoclonal

Isotype

IgG2b

Immunogen

Full length recombinant human SF3B4 expressed in and purified from E. coli

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06414 is reactive to SF3B4 in Human, Mouse, Rat

Applications

M06414 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.386kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000. IF/ICC and IHC: 1:1,000

Validation Images & Assay Conditions

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Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.

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Immunofluorescent analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The M06414 antibody reveals strong granular

Gene/Protein Information For SF3B4 (Source: Uniprot.org, NCBI)

Alternative Names

Hsh49; pre-mRNA splicing factor SF3b 49 kDa subunit; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49MGC10828; SF3b49; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); Spliceosome-associated protein 49; splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa SF3B4 AFD1, Hsh49, SAP49, SF3b49 splicing factor 3b subunit 4 splicing factor 3B subunit 4|SAP 49|SF3b50|pre-mRNA-splicing factor SF3b 49 kDa subunit|spliceosomal protein|spliceosome-associated protein (U2 snRNP)|spliceosome-associated protein 49|splicing factor 3b, subunit 4, 49kD|splicing factor 3b, subunit 4, 49kDa

For more info on SF3B4, check out the SF3B4 Infographic

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In this infographic, you will see the following information for SF3B4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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