Anti-SPOP Antibody

Product Name

Anti-SPOP Antibody

View all SPOP Antibodies

SKU/Catalog Number

A02032

Size

100μl

Form

Liquid

Description

Boster Bio Anti-SPOP Antibody catalog # A02032. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SPOP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02032)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from SPOP at AA range: 41-90.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02032 is reactive to SPOP in Human, Mouse, Rat

Applications

A02032 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

42.132kDa

Background of SPOP

MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase . MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.

Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-2000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of 3T3 HepG2 HeLa cells using SPOP Polyclonal antibody diluted at 1:1500.

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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.

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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.

Gene/Protein Information For SPOP (Source: Uniprot.org, NCBI)

Alternative Names

HIB homolog 1; Roadkill homolog 1; speckle-type POZ protein; TEF2 SPOP BTBD32, NEDMACE, NEDMIDF, NSDVS1, NSDVS2, TEF2 speckle type BTB/POZ protein speckle-type POZ protein|HIB homolog 1|roadkill homolog 1

For more info on SPOP, check out the SPOP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPOP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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